Cell culture article and methods thereof

• US 7,923,241 B2
• Filed: 10/10/2007
• Issued: 04/12/2011
• Est. Priority Date: 10/10/2007
• Status: Expired due to Fees

• Current Assignee: Corning Incorporated
• Original Assignee: Corning Incorporated
• Inventors: Peanasky, John S., Xi, Jun, Goodrich, Terry T., Gagnon, Paul E. Jr., Wang, Hongming, Lahiri, Joydeep, Fang, Ye
• Primary Examiner(s): Prouty; Rebecca E
• Assistant Examiner(s): Martin; Paul C
• Application Number:
  US11/973,832
• Publication Number:
  US 20090098645A1
• Time in Patent Office: 1,280 Days
• Field of Search: None
• US Class Current: 435/287.9
• CPC Class Codes: C12M 23/20 Material Coatings immunocoatings C12M25/00
• CPC Class Codes: C12M 25/06 Plates; Walls; Drawers; Multilayer plates

Abstract

Disclosed is a cell culture article including: a substrate; a tie-layer attached to at least the substrate; and a bio-compatible layer attached to at least the tie layer, the bio-compatible layer having been obtained from surface oxidation of a polymer layer. Also disclosed are methods for making the cell culture article and methods for performing an assay of a ligand with the article.

Claims

1. A cell culture article comprising:

a substrate;

a tie-layer attached to at least the substrate; and

a bio-compatible layer attached to at least the tie-layer,

the bio-compatible layer comprises a surface oxidation product of a polymer, the polymer comprising at least one oxidizable monomer, the polymer being selected from poly(styrene-maleic anhydride), poly(styrene-maleic acid), or a combination thereof, and the tie layer is aminopropylsilsesquioxane.

2. The article of claim 1, wherein the substrate comprises a plastic, a polymeric or co-polymeric substance, a ceramic, a glass, a metal, a crystalline material, a noble or semi-noble metal, a metallic or non-metallic oxide, a transition metal, or a combination thereof.

3. The article of claim 1, further comprising a bio-material associated with the bio-compatible layer.

4. The article of claim 3, wherein the bio-material comprises a natural or synthetic oligonucleotide, a natural or modified/blocked nucleotide/nucleoside, a nucleic acid (DNA) or (RNA), a natural peptide, a natural peptide comprising one or more modified or blocked amino acids, an antibody, a hapten, a biological ligand, a protein membrane, a lipid membrane, a protein, a small molecule, a cell, or a combination thereof, or a conjugate thereof.

5. The article of claim 1, wherein the substrate comprises at least one of an optical biosensor microplate, a microscope slide, an SPR plate, an impedance cell, or a combination thereof.

6. The article of claim 1, wherein the thickness of the biocompatible layer is from about 10 to about 1,000 Angstroms (Å

).

7. A method for preparing the cell culture article of claim 1, the method comprising:

providing a substrate having a tie-layer attached thereto, wherein the tie-layer is aminosilsesauioxane, and a polymer layer attached to the tie layer, the polymer being selected from poly(styrene-maleic anhydride), poly(styrene-maleic acid) or a combination thereof, the polymer comprising at least one oxidizable monomer; and

oxidizing the surface of the polymer layer to form a bio-compatible layer.

8. The method of claim 7, wherein oxidizing the surface of the polymer layer comprises contacting the surface of the polymer with UV-ozone, a plasma, or both.

9. The method of claim 7, further comprising associating a bio-material with the bio-compatible layer.

10. A method for performing an assay of a ligand, the method comprising:

contacting the ligand with a biosensor including at least one article of claim 1 and having a bio-material associated with the bio-compatible layer such that if the ligand binds to the bio-material, then;

detecting the ligand-induced response of the bio-material with the biosensor.

11. The method of claim 10, wherein the ligand-induced response is detected by a surface plasmon resonance biosensor, a waveguide resonant grating biosensor, an impedance biosensor, a mass spectrometry biosensor, or a combination thereof.

12. The method of claim 10, wherein the biomaterial comprises a live-cell.