Base sequence for control probe and method of designing the same

• US 7,923,546 B2
• Filed: 02/10/2006
• Issued: 04/12/2011
• Est. Priority Date: 02/14/2005
• Status: Expired due to Fees

• Current Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Original Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Inventors: Inui, Mimune
• Primary Examiner(s): Zhou; Shubo (Joe)
• Application Number:
  US11/350,947
• Publication Number:
  US 20060269933A1
• Time in Patent Office: 1,887 Days
• Field of Search: None
• US Class Current: 536/24.1
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence
• CPC Class Codes: G16B 25/00 ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
• CPC Class Codes: G16B 25/20 Polymerase chain reaction [PCR]; Primer or probe design; Probe optimisation

Abstract

There are provided a sequence for a control probe having a sequence rarely forming a secondary structure and rarely hybridizing with an undesired target and a method of designing thereof. There is further provided a probe carrier employing a control probe designed by the designing method. To provide these, a palindromic sequence is selected based on a sequence of which frequency is low in a database for a predetermined base sequence and used as the sequence of the control probe.

Claims

1. A control probe, comprising one base sequence heading from the center of the sequence of the control probe toward an end thereof and another base sequence heading from the center toward the other end thereof, wherein both base sequences are part of a base sequence which is identical when read from the 3′

side to the 5′

side and from the 5′

side to the 3′

side, and wherein the control probe comprises a base sequence represented by SEQ. ID No. 1.

2. A probe carrier, wherein the probe carrier is a solid phase carrier having a control probe according to claim 1 and a probe for detecting a target sequence contained in a specimen, and wherein both probes are immobilized on the solid phase carrier.

3. A nucleic acid detection kit, comprising the probe carrier according to claim 2 and an artificial nucleic acid having a complementary sequence to that of the control probe.