Software for the display of chromatographic separation data

• US 7,925,451 B2
• Filed: 04/16/2010
• Issued: 04/12/2011
• Est. Priority Date: 12/30/1997
• Status: Expired due to Fees

• Current Assignee: Caliper Life Sciences Incorporated (Revvity, Inc.)
• Original Assignee: Caliper Life Sciences Incorporated (Revvity, Inc.)
• Inventors: Gallagher, Steven J.
• Primary Examiner(s): Lau; Tung S
• Application Number:
  US12/761,787
• Publication Number:
  US 20100198526A1
• Time in Patent Office: 361 Days
• Field of Search: 702/32
• US Class Current: 702/32
• CPC Class Codes: G01N 2030/625 by measuring reference material, e.g. carrier without sample
• CPC Class Codes: G01N 27/44717 Arrangements for investigating the separated zones, e.g. localising zones
• CPC Class Codes: G01N 30/86 Signal analysis
• CPC Class Codes: G01N 30/8651 Recording, data aquisition, archiving and storage

Abstract

Techniques for displaying chromatographic data using a graphical user interface are provided. Chromatographic separation data that represent a series of measurements for multiple samples at a detection location over time can be displayed on a display device as a series of bands, the bands being arranged to resemble output from an electrophoresis gel. Additionally, the chromatographic separation data may be displayed in the form of measured intensity at the detection location versus time.

Claims

1. A method of acquiring and displaying chromatographic separation data, comprising:

acquiring data comprising a first series of measurements indicating presence of constituents and a marker in a first sample, the first sample constituents and the marker detected at a single point along the length of a channel as they move through the channel past that point;

acquiring data comprising a second series of measurements indicating presence of constituents and the marker in a second sample, the second sample constituents and the marker detected at the single point along the length of the channel as they move through the channel past that point;

displaying using a computer system the first series of measurements as a first lane comprising a first series of bands;

identifying one or more peaks in the first series of measurements that correspond to the marker;

identifying one or more peaks in the second series of measurements that correspond to the marker;

scaling the second series of measurements such that one or more bands that correspond to the marker in the first sample and one or more bands that correspond to the marker in the second sample are aligned when each series of measurements is displayed as a lane comprising a series of bands; and

displaying using the computer system the scaled second series of measurements as a second lane comprising a second series of bands, wherein the lane for the first series of measurements and the lane for the scaled second series of measurements are displayed as a parallel set of lanes.

2. The method of claim 1, wherein the second series of measurements is received after the first series of measurements.

3. The method of claim 1, wherein the series of measurements for each of the first and second samples includes peaks that indicate the presence of the constituents and the marker such that each peak corresponds to a displayed band.

4. The method of claim 1, wherein the first sample includes a standard ladder of macromolecular species.

5. The method of claim 1, further comprising:

displaying at least one of the first and second series of measurements as a graph of measurements vs. time.

6. The method of claim 1, further comprising:

displaying the one or more bands that correspond to the marker in each sample visually different from the bands that correspond to the constituents in each sample.

7. The method of claim 1, further comprising:

receiving input to invert the display of the first and second series of bands; and

inverting the displayed first and second series of bands.

8. The method of claim 1, wherein the separation system comprises a microfluidic device.

9. A computer program product that displays chromatographic separation data, comprising:

computer code that acquires data comprising a first series of measurements indicating presence of constituents and a marker in a first sample, the first sample constituents and the marker detected at a single point along the length of a channel as they move through the channel past that point;

computer code that acquires data comprising a second series of measurements indicating presence of constituents and the marker in a second sample, the second sample constituents and the marker detected at the single point along the length of the channel as they move through the channel past that point;

computer code that displays the first series of measurements as a first lane comprising a first series of bands;

computer code that identifies one or more peaks in the first series of measurements that correspond to the marker;

computer code that identifies one or more peaks in the second series of measurements that correspond to the marker;

computer code that scales the second series of measurements such that one or more bands that correspond to the marker in the first sample and one or more bands that correspond to the marker in the second sample are aligned when each series of measurements is displayed as a lane comprising a series of bands;

computer code that displays the scaled second series of measurements as a second lane comprising a second series of bands, wherein the lane for the first series of measurements and the lane for the scaled second series of measurements are displayed as a parallel set of lanes; and

a non-transitory, tangible computer readable medium that stores the computer codes.

10. The computer program product of claim 9, wherein the computer readable medium is selected from the group consisting of CD-ROM, floppy disk, tape, flash memory, system memory, and a hard drive.

11. The computer program product of claim 9, further comprising:

computer code that displays at least one of the first and second series of measurements as a graph of measurements vs. time.

12. The computer program product of claim 9, further comprising:

computer code that displays the one or more bands that correspond to the marker in each sample visually different from the bands that correspond to the constituents in each sample.

13. The computer program product of claim 9, wherein the separation system comprises a microfluidic device.

14. The computer program product of claim 9, wherein the series of measurements for each of the first and second samples includes peaks that indicate the presence of the constituents and the marker such that each peak corresponds to a displayed band.