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Method for linear mRNA amplification

  • US 7,927,838 B2
  • Filed: 07/11/2005
  • Issued: 04/19/2011
  • Est. Priority Date: 05/28/1999
  • Status: Expired due to Fees
First Claim
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1. A method for producing RNA, comprising:

  • (a) converting an RNA molecule to double-stranded cDNA, wherein one terminus of the double-stranded cDNA comprises an RNA polymerase promoter region; and

    (b) transcribing the double-stranded cDNA with an RNA polymerase and ribonucleotides in the presence of a reverse transcriptase that is incapable of RNA-dependent DNA polymerase activity during the transcribing step.

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