Methods and systems for in situ calibration of imaging in biological analysis
First Claim
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1. A method for validating the function of a biological instrument, the method comprising:
- providing an instrument comprising;
a photometric sensor having a plurality of pixels; and
a spatially non-uniform illumination source;
wherein the spatial non-uniformity of the illumination source comprises a range of signal intensities spanning the dynamic range of the photometric sensor;
capturing a series of images with the photometric sensor using the spatially non-uniform illumination source, wherein the images comprise a range of signal intensities spanning the dynamic range of the photometric sensor;
computing a value of a signal intensity and a noise level for each pixel, wherein each of the signal intensities of the range of spatially non-uniform signal intensities is associated with at least one pixel receiving the intensity;
estimating a relationship of signal intensity versus noise level for the photometric sensor over the dynamic range of the sensor using the computed values of signal intensity and noise level for each pixel; and
determining if the photometric detector is within the operational limits of detection using the estimated relationship.
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Abstract
Software, methods, and systems for calibrating photometric devices are provided. These involve using a non-uniform test illumination field to approximate a photon transfer curve by calculating stable pixel values and statistical dispersions on a pixel-by-pixel basis.
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12 Claims
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1. A method for validating the function of a biological instrument, the method comprising:
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providing an instrument comprising; a photometric sensor having a plurality of pixels; and a spatially non-uniform illumination source;
wherein the spatial non-uniformity of the illumination source comprises a range of signal intensities spanning the dynamic range of the photometric sensor;capturing a series of images with the photometric sensor using the spatially non-uniform illumination source, wherein the images comprise a range of signal intensities spanning the dynamic range of the photometric sensor; computing a value of a signal intensity and a noise level for each pixel, wherein each of the signal intensities of the range of spatially non-uniform signal intensities is associated with at least one pixel receiving the intensity; estimating a relationship of signal intensity versus noise level for the photometric sensor over the dynamic range of the sensor using the computed values of signal intensity and noise level for each pixel; and determining if the photometric detector is within the operational limits of detection using the estimated relationship. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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Specification