Uniform surfaces for hybrid material substrates and methods for making and using same

• US 7,931,867 B2
• Filed: 10/31/2007
• Issued: 04/26/2011
• Est. Priority Date: 11/28/2005
• Status: Active Grant

• Current Assignee: Pacific Bioscience of California Incorporated (L'Oreal SA)
• Original Assignee: Pacific Bioscience of California Incorporated (L'Oreal SA)
• Inventors: Korlach, Jonas
• Primary Examiner(s): Alexander; Lyle A
• Assistant Examiner(s): WHITE, DENNIS MICHAEL
• Application Number:
  US11/981,638
• Publication Number:
  US 20080145278A1
• Time in Patent Office: 1,273 Days
• Field of Search: 422/50, 422/82.05, 422/82.08, 422/82.09, 422/82.11, 422/98, 422/425, 422/426, 422/57, 435/4, 435/6, 435/287.2, 435/288.4, 435/288.5, 435/288.7, 204/403.01, 204/403.07, 204/418, 204/643, 205/188, 205/777.5, 205/201.3, 205/208.1, 205/216, 205/225, 205/251, 205/353, 205/492.1, 257/253, 257/414, 257/750, 324/71.1, 359/230, 359/245, 359/318, 359/330, 359/346, 385/12, 385/31, 385/37, 385/116, 385/122, 385/126, 385/129, 385/132, 427/437, 428/215, 430/317, 430/321, 436/164, 436/518, 436/531, 438/1, 438/48, 438/71, 438/116, 438/118, 438/618, 506/16, 506/18, 600/377, 720/719
• US Class Current: 422/82.08
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00576 fluorophore
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00619 using hydrophilic or hydrophobic regions
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/00635 by reactive plasma treatment
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01L 3/5088 confining liquids at a location by surface tension, e.g. virtual wells on plates, wires B01L3/50857 takes precedence
• CPC Class Codes: G01N 21/0303 Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment G01N21/031 - G01N21/15 take precedence
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 21/648 using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
• CPC Class Codes: G01N 21/7703 using reagent-clad optical fibres or optical waveguides using measurement of total internal reflection or attenuated total reflection G01N21/552; optical fibres or waveguides per se G02B
• CPC Class Codes: Y10T 428/24942 including components having same physical characteristic in differing degree
• CPC Class Codes: Y10T 428/24967 Absolute thicknesses specified
• CPC Class Codes: Y10T 428/31612 As silicone, silane or siloxane

Abstract

Devices, systems and methods of using same where hybrid substrate materials are provided with a substantially uniform surface to provide uniformity of properties, including interaction with their environments. Uniform surfaces are applied as coatings over, e.g., hybrid metal/silica, metal/polymer, metal/metal surfaces to mask different chemical properties of differing regions of the surface and to afford a protective surface for the hybrid structure.

Claims

1. A system, comprising:

a substrate, comprising;

a first layer comprising a first surface having at least first and second material regions, the first and second material regions having one or more chemical or physical properties that differ when the first and second regions are exposed to a first chemical environment, wherein said first material region comprises a transparent material, and said second material comprises an opaque metal with any associated metal oxide;

wherein the first layer comprises a layer of the first material and a layer of the second material, wherein the layer of second material is a discontinuous layer disposed over the layer of first material layer resulting in a first surface of the first layer having apertures with exposed regions of first material, the apertures having a cross-sectional dimension that is from about 10 nm to about 200 nm;

a second layer, comprising a coating material that associates preferentially with the second material regions, disposed selectively over second material regions to provide a second surface to the substrate, whereby the coating material covers substantially all of the second material regions, whereby at least portions of the first material regions exposed by the aperture are not coated by the second layer, and wherein the second surface that is substantially uniform with respect to the one or more chemical or physical properties, when the second surface is exposed to the first chemical environment; and

an optical detection system positioned to receive optical signals from a reaction fluid disposed upon the substrate.

2. The system of claim 1, wherein the optical detection system comprises an excitation light source positioned to direct excitation light at the reaction fluid, and a fluorescence detector for detecting fluorescent signals from the reaction fluid.

3. The system of claim 1, wherein the one or more physical or chemical properties are selected from chemical composition, surface charge, relative hydrophobicity, conductivity, oxidative capacity, surface roughness, and density of reactive chemical moieties.

4. The system of claim 1, wherein the first material comprises a silica based material.

5. The system of claim 4, wherein the first material is selected from glass, quartz, and fused silica.

6. The system of claim 1, wherein the apertures have a cross-sectional dimension that is from about 20 nm to about 100 nm.

7. The system of claim 1, wherein the second layer comprises an inorganic layer.

8. The system of claim 7, wherein the inorganic layer comprises a silica based layer.

9. The system of claim 8, wherein the silica based layer comprises silicon oxide.

10. The system of claim 1, wherein the second layer comprises an organic layer.

11. The system of claim 10, wherein the organic layer comprises organic materials selected from organic polymers, organosilicates, and organosilanes.