Compositions and methods for the preparation of human growth hormone glycosylation mutants
First Claim
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1. An isolated human growth hormone polypeptide comprising the amino acid sequence of SEQ ID NO:
- 1 or SEQ ID NO;
2 except for the presence of at least one O-linked glycosylation site not present in SEQ ID NO;
1 or SEQ ID NO;
2, wherein the O-linked glycosylation site is a serine or threonine residue, and wherein the O-linked glycosylation site is present at a site selected from the group consisting of (a) proximate a proline residue located at position 2, 5, 37, 48, 59, 89, or 133 of SEQ ID NO;
1 or SEQ ID NO;
2, (b) the amino acid sequence surrounding position 140 of SEQ ID NO;
1 or SEQ ID NO;
2, and (c) the amino acid sequence at the C-terminus of SEQ ID NO;
1 or SEQ ID NO;
2.
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Abstract
The present invention relates to mutants of human growth hormone, which contain newly introduced N-linked or O-linked glycosylation site(s), such that these recombinantly produced polypeptides have glycosylation patterns distinctly different from that of the naturally occurring human growth hormone. The polynucleotide coding sequences for the mutants, expression cassettes comprising the coding sequences, cells expressing the mutants, and methods for producing the mutants are also disclosed. Further disclosed are pharmaceutical compositions comprising the mutants and method for using the mutants.
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Citations
33 Claims
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1. An isolated human growth hormone polypeptide comprising the amino acid sequence of SEQ ID NO:
- 1 or SEQ ID NO;
2 except for the presence of at least one O-linked glycosylation site not present in SEQ ID NO;
1 or SEQ ID NO;
2, wherein the O-linked glycosylation site is a serine or threonine residue, and wherein the O-linked glycosylation site is present at a site selected from the group consisting of (a) proximate a proline residue located at position 2, 5, 37, 48, 59, 89, or 133 of SEQ ID NO;
1 or SEQ ID NO;
2, (b) the amino acid sequence surrounding position 140 of SEQ ID NO;
1 or SEQ ID NO;
2, and (c) the amino acid sequence at the C-terminus of SEQ ID NO;
1 or SEQ ID NO;
2. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33)
- 1 or SEQ ID NO;
Specification