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Sequence determination of nucleic acids using electronic detection

  • US 7,935,481 B1
  • Filed: 07/26/2000
  • Issued: 05/03/2011
  • Est. Priority Date: 07/26/1999
  • Status: Active Grant
First Claim
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1. A method of determining the identification of nucleotide(s) at a first detection position in a first domain of a target sequence, said target sequence comprising said first domain and a second domain, said method comprising:

  • a. providing an electrode with a covalently attached capture probe, wherein said capture probe has a sequence substantially complementary to said second domain of said target sequence;

    b. contacting said electrode with;

    (i) said target sequence;

    (ii) a first label probe substantially complementary to said first domain, comprising a first nucleotide at an interrogation position and a first electron transfer moiety (ETM) with a first redox potential;

    (iii) a second label probe substantially complementary to said first domain, comprising a second nucleotide at said interrogation position and a second ETM with a second redox potential;

    under conditions wherein if said nucleotide at said interrogation position is perfectly complementary to said detection position, hybridization of said probe(s) occurs; and

    c. detecting the presence of said first and/or second ETM to determine the nucleotide(s) at said detection position.

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