Methods of detecting early renal disease in animals
First Claim
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1. A method to detect early renal disease comprising:
- (a) obtaining a sample from an animal;
(b) applying at least part of the sample to a device capable of detecting albumin in the sample; and
(c) obtaining a urinary albumin concentration from the device,wherein an amount of albumin in a range from about 10 μ
g/ml to about 300 μ
g/ml in the sample, when the specific gravity of the sample is normalized to 1.010 g/ml, is indicative of early renal disease.
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Abstract
The present invention provides a method for the detection of early renal disease in animals. The method includes the steps of (a) obtaining a sample from an animal to be tested and (b) determining the amount of albumin in the sample. An amount of albumin in the range of from 10 μg/ml to about 300 μg/ml indicates the presence of early renal disease. The present invention also provides antibodies to canine, feline and equine albumin which can be used to detect the presence of early renal disease.
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Citations
20 Claims
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1. A method to detect early renal disease comprising:
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(a) obtaining a sample from an animal; (b) applying at least part of the sample to a device capable of detecting albumin in the sample; and (c) obtaining a urinary albumin concentration from the device, wherein an amount of albumin in a range from about 10 μ
g/ml to about 300 μ
g/ml in the sample, when the specific gravity of the sample is normalized to 1.010 g/ml, is indicative of early renal disease. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method to identify an animal at risk for developing late-stage renal disease comprising:
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(a) obtaining a sample from an animal; (b) applying at least part of the sample to a device capable of detecting albumin in the sample; and (c) obtaining a urinary albumin concentration from the device, wherein an amount of albumin in a range from about 10 μ
g/ml to about 300 μ
g/ml in the sample, when the specific gravity of the sample is normalized to 1.010 g/ml, is indicative of early renal disease. - View Dependent Claims (11, 12, 13)
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14. A method to detect early renal disease comprising
(a) obtaining a sample of urine from an animal; -
(b) applying at least part of the sample to a device comprising a support structure defining a capillary flow path, the support structure comprising; (i) a sample pad for receiving the sample; (ii) a labeling area fluidly connected to the sample pad, the labeling area comprising a labeling reagent that comprises an albumin-binding compound conjugated to a detectable marker; (iii) a first capture area fluidly connected to the labeling area, the first capture area comprising an immobilized first capture reagent that binds the albumin-binding compound/detectable marker conjugate, wherein the amount of immobilized first capture reagent is sufficient to capture 50% of the conjugate when the concentration of albumin in the sample is less than 10 ug/ml; (iv) a second capture area fluidly connected to the first capture area, the second capture area comprising an immobilized second capture reagent for capturing albumin-binding compound/detectable marker conjugate not captured by the first capture regent; (c) comparing the amount of detectable marker in the first and second capture areas; wherein a greater relative detectable marker intensity in the second capture area as compared to the detectable marker intensity in the first capture area indicates the presence of early renal disease. - View Dependent Claims (15, 16, 17, 18, 19, 20)
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Specification