Thermal cycling methods

• US 7,935,504 B2
• Filed: 11/15/2005
• Issued: 05/03/2011
• Est. Priority Date: 12/29/2000
• Status: Expired due to Fees

• Current Assignee: Roche Molecular Systems (Roche Holding AG)
• Original Assignee: CHEN Ching-Too
• Inventors: Chen, Shuqi
• Primary Examiner(s): Beisner; William H
• Assistant Examiner(s): HOBBS, MICHAEL L
• Application Number:
  US11/280,801
• Publication Number:
  US 20060154341A1
• Time in Patent Office: 1,995 Days
• Field of Search: 435/304.2, 435/809, 435/6, 435/91.2
• US Class Current: 435/91.2
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0832 cylindrical, tube shaped
• CPC Class Codes: B01L 2300/0874 Three dimensional network
• CPC Class Codes: B01L 2300/1822 using Peltier elements
• CPC Class Codes: B01L 2300/1861 using radiation
• CPC Class Codes: B01L 2400/0421 electrophoretic flow
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 2400/0655 pinch valves
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/5082 Test tubes per se
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: G01N 35/1002 Reagent dispensers
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A thermal cycling method may include adding a sample to a sample vessel, placing the sample vessel into a thermal cycler having a processing unit with three processing stations, compressing the sample vessel with the first compression member, transferring energy to the sample with the second energy transfer element, transferring energy to the sample with the third energy transfer element, compressing the sample vessel with the third compression member, and transferring energy to the sample with the first energy transfer element.

Claims

1. A method of thermal cycling, comprising:

adding a sample to a sample vessel that comprises;

a sample-containing portion defined by at least one wall and having;

an interface to receive at least one sample, and

at least a first segment, a second segment, and a third segment distal to the interface;

the first segment being in fluid communication with the interface and being so thermally conductive as to permit the transfer of thermal energy to and from the contents of the first segment, so compressible as to permit opposing wall portions of the first segment to touch one another when the first segment is compressed, and so expandable as to receive a volume of fluid expelled from the second segment when not so compressed;

the second segment being so thermally conductive as to permit the transfer of thermal energy to and from the contents of the second segment, so compressible as to permit opposing wall portions of the second segment to touch one another when the second segment is compressed, and so expandable as to receive a volume of fluid expelled from the first segment; and

the third segment being so thermally conductive as to permit the transfer of thermal energy to and from the contents of the third segment, so compressible as to permit opposing wall portions of the third segment to touch one another when the third segment is compressed, and so expandable as to receive a volume of fluid expelled from the second segment;

introducing the sample vessel into a thermal cycler, the thermal cycler comprising

a processing unit having an opening to receive the sample vessel, the processing unit having a first processing station, a second processing station, and a third processing station positioned along the opening,

the first processing station including a first compression member adapted to compress the sample vessel within the opening and a first energy transfer element for transferring energy to the sample at the first processing station,

the second processing station including a second compression member adapted to compress the sample vessel within the opening and a second energy transfer element for transferring energy to the sample at the second processing station, and

the third processing station including a third compression member adapted to compress the sample vessel within the opening and a third energy transfer element for transferring energy to the sample at the third processing station;

wherein compression of the sample vessel by of one of the compression members displaces the sample within the sample vessel between the processing stations;

compressing the sample vessel with the first compression member;

transferring energy to the sample with the second energy transfer element;

transferring energy to the sample with the third energy transfer element;

compressing the sample vessel with the third compression member; and

transferring energy to the sample with the first energy transfer element.

2. The method of claim 1, further comprising compressing the sample vessel with the second compression member.

3. The method of claim 1, further comprising partially compressing the sample vessel with the second compression member.

4. The method of claim 1, further comprising heating the sample in the first processing station to a first temperature.

5. The method of claim 4, further comprising heating the sample in the second processing station to a second temperature.

6. The method of claim 5, further comprising heating the sample in the third processing station to a third temperature.

7. The method of claim 6, wherein the second temperature is effective to denature nucleic acid in the sample and the first and/or third temperature is one at which nucleic acid annealing and nucleic acid synthesis can occur.

8. The method of claim 7, wherein the first temperature is different from the third temperature.

9. The method of claim 1, further comprising analyzing the sample by detecting a signal from the sample, and analyzing the detected signal to determine a condition of the sample.

10. The method of claim 9, wherein analyzing further comprises applying an excitation energy to the sample.

11. The method of claim 9, wherein the signal from the sample is detected in at least one of the first processing station and the third processing station.

12. The method of claim 11, wherein the signal from the sample is detected in only one of the first processing station and the third processing station.