Indicator immobilization on assay devices
First Claim
1. A lateral flow assay device for detecting the presence or quantity of an analyte in a test sample comprising a chromatographic medium that includes a conjugate pad located upstream of a detection zone, wherein a crosslinked network is non-diffusively immobilized within the detection zone, the crosslinked network containing a plurality of small molecule indicators that are configured to undergo a detectable color change in the presence of the analyte, the small molecule indicators of the crosslinked network being crosslinked to one another either directly or via a crosslinking agent that binds the small molecule indicators, the crosslinked network further comprising an anchoring compound that links the chromatographic medium and one of the plurality of small molecular indicators of the crosslinked network.
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Abstract
A lateral flow assay device for detecting the presence or quantity of an analyte in a test sample is provided. The device comprises a chromatographic medium that defines a detection zone, wherein a crosslinked network is non-diffusively immobilized within the detection zone. The crosslinked network contains a small molecule indicator that is configured to undergo a detectable color change in the presence of the analyte.
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15 Claims
- 1. A lateral flow assay device for detecting the presence or quantity of an analyte in a test sample comprising a chromatographic medium that includes a conjugate pad located upstream of a detection zone, wherein a crosslinked network is non-diffusively immobilized within the detection zone, the crosslinked network containing a plurality of small molecule indicators that are configured to undergo a detectable color change in the presence of the analyte, the small molecule indicators of the crosslinked network being crosslinked to one another either directly or via a crosslinking agent that binds the small molecule indicators, the crosslinked network further comprising an anchoring compound that links the chromatographic medium and one of the plurality of small molecular indicators of the crosslinked network.
Specification