Transgenic animals secreting desired proteins into milk

• US 7,939,317 B1
• Filed: 02/20/1992
• Issued: 05/10/2011
• Est. Priority Date: 04/09/1986
• Status: Active Grant

• Current Assignee: Genzyme Corporation (Sanofi )
• Original Assignee: Genzyme Corporation (Sanofi )
• Inventors: Gordon, Katherine, Groet, Suzanne
• Primary Examiner(s): Paras, Jr.; Peter
• Assistant Examiner(s): MONTANARI, DAVID A
• Application Number:
  US07/839,194
• Time in Patent Office: 7,019 Days
• Field of Search: 435/320.1, 435/172.3, 536/27, 536/24.1, 536/23.1, 800/2, 800/DIG.1, 800/4, 800/14, 800/15, 800/16, 800/17, 800/18, 935/22, 935/48, 935/111
• US Class Current: 435/320.1
• CPC Class Codes: A01K 2207/15 Humanized animals
• CPC Class Codes: A01K 2217/00 Genetically modified animals
• CPC Class Codes: A01K 2217/05 Animals comprising random inserted nucleic acids (transgenic)
• CPC Class Codes: A01K 2227/10 Mammal
• CPC Class Codes: A01K 2267/01 Animal expressing industrially exogenous proteins
• CPC Class Codes: C07K 14/005 from viruses
• CPC Class Codes: C12N 15/85 for animal cells
• CPC Class Codes: C12N 15/8509 for producing genetically modified animals, e.g. transgenic
• CPC Class Codes: C12N 15/89 using microinjection
• CPC Class Codes: C12N 2730/10122 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2830/008 cell type or tissue specific enhancer/promoter combination
• CPC Class Codes: C12N 2830/85 mammalian
• CPC Class Codes: C12N 9/6459 t-plasminogen activator (3.4.21.68), i.e. tPA
• CPC Class Codes: C12Y 304/21069 Protein C activated (3.4.21.69)

Abstract

A DNA sequence containing a gene encoding a protein, the gene being under the transcriptional control in the DNA sequence of a mammalian milk protein promoter which does not naturally control the transcription of the gene, such DNA sequence including DNA enabling secretion of the protein.

Claims

1. A DNA construct comprising a gene encoding a protein, said gene being under transcriptional control of a mammalian milk protein promoter sequence which does not naturally control transcription of said gene, said DNA construct further comprising DNA encoding a peptide enabling secretion of said protein.

2. The DNA construct of claim 1, wherein said secretion enabling peptide comprises a secretion signal peptide which is cleaved from said secretion protein.

3. The DNA construct of claim 1, wherein said DNA encoding a peptide enabling secretion of said protein is the signal encoding sequence naturally associated with said gene encoding said protein.

4. The DNA construct of claim 1, wherein said DNA encoding a peptide enabling secretion of said protein is the signal encoding sequence naturally associated with said mammalian milk protein promoter.

5. The DNA construct of claim 1, wherein said DNA sequence includes a transcriptional stop sequence.

6. The DNA construct of claim 5, wherein said stop sequence comprises the SV40 virus polyadenylation site.

7. The DNA construct of claim 1, wherein said gene encodes human tissue plasminogen activator or hepatitis B surface antigen.

8. The DNA construct of claim 1, wherein said milk protein is a milk serum protein.

9. The DNA construct of claim 8, wherein said milk serum protein is alpha-lactalbumin.

10. The DNA construct of claim 1, wherein the mammalian milk protein promoter sequence is the sequence of a milk serum protein promoter.

11. The DNA construct of claim 10, wherein the milk serum protein promoter is an α

-lactalbumin promoter.

12. The DNA construct of claim 10, wherein the milk serum protein promoter is a β

-lactoglobulin promoter.

13. The DNA construct of claim 1, wherein the mammalian milk protein promoter sequence is the sequence of a casein protein promoter.