Reaction buffer composition for nucleic acid replication with packed DNA polymerases
First Claim
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1. A composition for nucleic acid replication, comprising a mineral salt, ammonium sulfate, and at least one fusion DNA polymerase, said composition having a mineral salt:
- ammonium sulfate molar ratio in the range from about 30;
1 to 240;
1,wherein the DNA polymerase(s) concentration is at least 6 units per 50 μ
l.
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Abstract
The invention relates to compositions, methods, and kits for nucleic acid replication, including polymerase chain reaction (PCR) and mutagenesis reactions. A buffer composition is provided which allows higher concentrations of DNA polymerase to be used, resulting in greater yield of amplified product and faster reaction kinetics.
54 Citations
50 Claims
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1. A composition for nucleic acid replication, comprising a mineral salt, ammonium sulfate, and at least one fusion DNA polymerase, said composition having a mineral salt:
- ammonium sulfate molar ratio in the range from about 30;
1 to 240;
1,wherein the DNA polymerase(s) concentration is at least 6 units per 50 μ
l. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50)
- ammonium sulfate molar ratio in the range from about 30;
Specification