Methods of in-vitro analysis using time-domain NMR spectroscopy
First Claim
1. An in vitro method of determining an analyte concentration of a sample, the method comprising the acts of:
- adding an NMR contrast agent to the sample to enhance detection of the analyte;
placing the sample into a low-field, bench-top time-domain nuclear magnetic resonance (TD-NMR) spectrometer, the NMR spectrometer being tuned to measure a selected type of atom;
applying a magnetic field to the sample;
applying at least one radio-frequency pulse to the sample;
removing the radio-frequency pulse from the sample so as to produce a decaying NMR signal;
measuring the decaying NMR signal; and
calculating the analyte concentration from the measurement associated with the decaying NMR signal and a selected model.
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Abstract
An in vitro method of determining an analyte concentration of a sample includes placing the sample into a low-field, bench-top time-domain nuclear magnetic resonance (TD-NMR) spectrometer. The NMR spectrometer is tuned to measure a selected type of atom. A magnetic field is applied to the sample using a fixed, permanent magnet. At least one 90 degree radio-frequency pulse is applied to the sample. The radio-frequency pulse is generally perpendicular to the magnetic field. The 90 degree radio-frequency pulse is removed from the sample so as to produce a decaying NMR signal. The decaying NMR signal is measured at a plurality of times while applying a plurality of 180 degree refocusing radio-frequency pulses to the sample. The analyte concentration is calculated from the plurality of measurements associated with the decaying NMR signal and a selected model.
21 Citations
20 Claims
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1. An in vitro method of determining an analyte concentration of a sample, the method comprising the acts of:
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adding an NMR contrast agent to the sample to enhance detection of the analyte; placing the sample into a low-field, bench-top time-domain nuclear magnetic resonance (TD-NMR) spectrometer, the NMR spectrometer being tuned to measure a selected type of atom; applying a magnetic field to the sample; applying at least one radio-frequency pulse to the sample; removing the radio-frequency pulse from the sample so as to produce a decaying NMR signal; measuring the decaying NMR signal; and calculating the analyte concentration from the measurement associated with the decaying NMR signal and a selected model. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. An in vitro method of determining an analyte concentration of a sample, the method comprising the acts of:
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placing the sample into a low-field, bench-top time-domain nuclear magnetic resonance (TD-NMR) spectrometer, the NMR spectrometer being tuned to measure a selected type of atom; applying a magnetic field to the sample; applying at least one radio-frequency pulse to the sample; removing the radio-frequency pulse from the sample so as to produce a decaying NMR signal; measuring the decaying NMR signal; and calculating the analyte concentration from the measurement associated with the decaying NMR signal and a multivariate calibration model. - View Dependent Claims (10, 11, 12, 13)
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14. A method of determining an analyte concentration of a sample, the method comprising the acts of:
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developing a multivariate calibration model by measuring NMR relaxation for a plurality of calibration samples; measuring a NMR relaxation for a sample with a nuclear magnetic resonance (NMR) spectrometer; comparing the measurement of the sample to the multivariate calibration model to identify the differences between the calibration model and the measurement of the test sample; and calculating the analyte concentration from the comparison of the sample to the multivariate calibration model. - View Dependent Claims (15, 16, 17, 18, 19, 20)
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Specification