Valves and pumps for microfluidic systems and method for making microfluidic systems

• US 7,942,160 B2
• Filed: 06/14/2004
• Issued: 05/17/2011
• Est. Priority Date: 01/08/2001
• Status: Active Grant

• Current Assignee: President and Fellows of Harvard College (Harvard Corporation)
• Original Assignee: President and Fellows of Harvard College (Harvard Corporation)
• Inventors: Metallo, Steven J., McDonald, J. Cooper, Choi, Insung S., Stone, Howard A., Wu, Hongkai, Anderson, Janelle R., Chiu, Daniel T., Wargo, Christopher J., Whitesides, George M., Jeon, Noo Li
• Primary Examiner(s): Lee; Kevin L
• Application Number:
  US10/867,151
• Publication Number:
  US 20040228734A1
• Time in Patent Office: 2,528 Days
• Field of Search: 251/331, 137/613, 137/843, 137/859, 137/533.11, 137/15.19, 137/855
• US Class Current: 137/15.19
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/0611 duck bill valves
• CPC Class Codes: B01L 2400/0638 membrane valves, flap valves
• CPC Class Codes: B01L 2400/0677 phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: F04B 43/043 Micropumps
• CPC Class Codes: F16K 15/144 the closure elements being fixed along all or a part of their periphery
• CPC Class Codes: F16K 15/16 with tongue-shaped laminae
• CPC Class Codes: F16K 2099/0074 using photolithography, e.g. etching
• CPC Class Codes: F16K 2099/0078 using moulding or stamping
• CPC Class Codes: F16K 2099/0084 Chemistry or biology, e.g. "lab-on-a-chip" technology
• CPC Class Codes: F16K 2099/0086 Medical applications
• CPC Class Codes: F16K 2099/0094 Micropumps
• CPC Class Codes: F16K 99/0001 Microvalves microdevices B81B1/00; manufacture or treatment of devices or systems in or on a substrate B81C1/00; microfluidic structures B01L3/5027; micropumps F04B19/006
• CPC Class Codes: F16K 99/0007 of cantilever type
• CPC Class Codes: F16K 99/0015 : Diaphragm or membrane valves
• CPC Class Codes: F16K 99/003 : Valves for single use only
• CPC Class Codes: F16K 99/0034 : Operating means specially adapted for microvalves
• CPC Class Codes: F16K 99/0048 : using piezoelectric means
• CPC Class Codes: F16K 99/0057 : the fluid being the circulating fluid itself, e.g. check valves
• CPC Class Codes: F16K 99/0059 : actuated by a pilot fluid
• CPC Class Codes: Y10T 137/0497 : Fluid actuated or retarded
• CPC Class Codes: Y10T 137/7891 : Flap or reed
• CPC Class Codes: Y10T 137/791 : Ball valves
• CPC Class Codes: Y10T 137/87917 : Flow path with serial valves and/or closures

Abstract

The present invention relates to microfluidic systems, including valves and pumps for microfluidic systems. The valves of the invention include check valves such as diaphragm valves and flap valves. Other valves of the invention include one-use valves. The pumps of the present invention include a reservoir and at least two check valves. The reservoir may be of variable volume. The present invention also relates to a flexible microfluidic system. The present invention additionally relates to a method of making microfluidic systems including those of the present invention. The method includes forming a microfluidic system on a master, connecting a support to the microfluidic system and removing the microfluidic system from the master. The support may remain connected to the microfluidic system or the microfluidic system may be transferred to another substrate. The present invention further relates to a method of manipulating a flow of a fluid in a microfluidic system. This method includes initiating fluid flow in a first direction and inhibiting fluid flow in a second direction and may be practiced with the valves of the present invention.

Claims

1. A microfluidic system comprising:

a fluid path;

an inlet to the fluid path;

an outlet to the fluid path;

a first closing member disposed along the fluid path between the inlet and the outlet, wherein the first closing member comprises a check valve;

a second closing member disposed along the fluid path between the inlet and the outlet; and

a reservoir disposed along the fluid path between the first closing member and the second closing member,

wherein the fluid path has a cross-sectional dimension of less than about 500 μ

m, and

wherein the fluid path is formed in a polymeric material.

2. The microfluidic system of claim 1, wherein the first closing member comprises a first flexible member.

3. The microfluidic system of claim 2, wherein the first closing member has an open position and a closed position and is constructed and arranged to travel from the closed position to the open position without sliding against any portion of the microfluidic system.

4. The microfluidic system of claim 2, wherein the first flexible member comprises a flap.

5. The microfluidic system of claim 4, wherein the flap is generally semicircular in shape.

6. The microfluidic system of claim 2, wherein the first flexible member comprises a diaphragm.

7. The microfluidic system of claim 6, wherein the diaphragm comprises an opening.

8. The microfluidic system of claim 7, further comprising a seat constructed and arranged to support the diaphragm around at least the periphery of the opening.

9. The microfluidic system of claim 2, wherein the first flexible member comprises an elastomer.

10. The microfluidic system of claim 1, wherein the first closing member comprises a first free-floating member.

11. The microfluidic system of claim 10, wherein the first free-floating member comprises a spherical body.

12. The microfluidic system of claim 1, wherein the fluid path is constructed of a flexible material.

13. The microfluidic system of claim 12, wherein the fluid path is constructed of an elastomer.

14. The microfluidic system of claim 12, further comprising:

a flexible support connected to the flexible material.

15. The microfluidic system of claim 1, wherein a volume of the reservoir is variable.

16. The microfluidic system of claim 1, wherein the reservoir comprises an elastomer.

17. The microfluidic system of claim 1, wherein the fluid path has a cross-sectional dimension of less than about 300 μ

m.

18. The microfluidic system of claim 17, wherein the fluid path has a cross-sectional dimension of less than about 100 μ

m.

19. The microfluidic system of claim 18, wherein the fluid path has a cross-sectional dimension of less than about 50 μ

m.

20. The microfluidic system of claim 1, wherein at least one of the first and second closing members comprises a substantially sealed barrier and further comprising an electrode electrically connected to at least one of the inlet of the fluid path and the outlet of the fluid path.

21. The microfluidic system of claim 1, wherein the first closing member has an open position and a closed position and is constructed and arranged to travel from the closed position to the open position or from the open position to the closed position by the flow of a fluid in the fluid path from the inlet to the outlet.

22. The microfluidic system of claim 21, further comprising a flexible support connected to the fluid path.

23. The microfluidic system of claim 22, wherein the flexible support is an adhesive tape.

24. A method for opening the check valve of the microfluidic system of claim 1, comprising:

providing a flow of a fluid through the fluid path; and

deflecting the first closing member with the flow from a closed position to an open position without the first closing member sliding against any portion of the check valve.

25. A method for manipulating a flow of a fluid in the microfluidic system of claim 1, comprising:

initiating the flow of the fluid through the fluid path in a first direction; and

inhibiting the flow of the fluid through the fluid path in a second direction.

26. A microfluidic system as in claim 1, wherein the first closing member has an open position and a closed position and is constructed and arranged to travel from the from the open position to the closed position by the flow of a fluid in the fluid path from the inlet to the outlet.

27. A microfluidic system as in claim 1, wherein the first closing member has an open position and a closed position and comprises a flap disposed along the fluid path between the inlet and the outlet, the microfluidic system further comprising a seat constructed and arranged to support the flap such that the flap rests on the seat in the closed position.

28. A microfluidic system as in claim 1, wherein the first closing member has an open position and a closed position, and is positioned in the closed position at rest.

29. A microfluidic system as in claim 1, wherein the first closing member has an open position and a closed position, and is positioned in the open position at rest.

30. A microfluidic system as in claim 1, wherein the first closing member comprises two or more flaps.

31. A microfluidic system as in claim 30, wherein the two or more flaps are constructed and arranged to close against one another.