Polynucleotides for use as tags and tag complements, manufacture and use thereof

• US 7,943,322 B2
• Filed: 10/27/2010
• Issued: 05/17/2011
• Est. Priority Date: 01/25/2001
• Status: Expired due to Fees

• Current Assignee: Luminex Molecular Diagnostics Incorporated (DiaSorin S.p.A.)
• Original Assignee: Luminex Molecular Diagnostics Incorporated (DiaSorin S.p.A.)
• Inventors: Benight, Albert S., Bullock, Richard S., Janota, Vit, Kobler, Daniel, Pancoska, Petr, Fieldhouse, Daniel, Riccelli, Peter V.
• Primary Examiner(s): Martinell; James
• Application Number:
  US12/912,827
• Publication Number:
  US 20110039726A1
• Time in Patent Office: 202 Days
• Field of Search: None
• US Class Current: 435/6.11
• CPC Class Codes: C12Q 1/6855 Ligating adaptors
• CPC Class Codes: C12Q 1/6876 Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
• CPC Class Codes: Y10T 436/13 Tracers or tags
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

A family of minimally cross-hybridizing nucleotide sequences, methods of use, etc. A specific family of 210 24 mers is described.

Claims

1. An oligonucleotide tag or tag complement for use in a multiplex assay, wherein the tag or tag complement is selected from the group of oligonucleotides consisting of:

TGATATAGATAGTTAGATAGATAG (SEQ ID NO;

     104),

ATGATGATGTATTGTAGTTATGAA (SEQ ID NO;

     105),

GTTAGTTAGATTATTGTTAGTTAG (SEQ ID NO;

     107),

ATTGTTAGAAAGTGTAGATTAAAG (SEQ ID NO;

     110),

ATGAGTATGTTATTAGTGTATGTA (SEQ ID NO;

     111),

TGTAATAGTGAAGTTAGATTGTAT (SEQ ID NO;

     112),

ATTGATAGATGATTAGTTAGTTGA (SEQ ID NO;

     113),

TGATGTTTGATTATGATGTAGTAT (SEQ ID NO;

     115),

ATTGTTAGTGATGTTAGTAATTAG (SEQ ID NO;

     117), and

TGATGTAAGTATTGATGTTAGTTT (SEQ ID NO;

     118),

including oligonucleotides complementary thereto.

2. The oligonucleotide of claim 1, wherein one of the oligonucleotides in SEQ ID NOs:

104, 105, 107, 110-113, 115, 117, and 118 does not cross-hybridize with a second, different oligonucleotide in SEQ ID NOs;

104, 105, 107, 110-113, 115, 117, and 118 under the following hybridization conditions;

0.2 M NaCl, 0.1 M Tris, 0.08% Triton X-100, pH 8.0 at 37°

C.

3. The oligonucleotide of claim 1, wherein the oligonucleotide is attached to a solid support.

4. The oligonucleotide of claim 3, wherein the solid support is a microparticle.

5. An improved method of analyzing a biological sample suspected of comprising a target, wherein the improvement comprises using the oligonucleotide tag or tag complement of claim 1.

6. A nucleic acid tag or tag complement comprising an oligonucleotide selected from the group of oligonucleotides consisting of:

SEQ ID NO;

104, SEQ ID NO;

105, SEQ ID NO;

107, SEQ ID NO;

110, SEQ ID NO;

111, SEQ ID NO;

112, SEQ ID NO;

113, SEQ ID NO;

115, SEQ ID NO;

117, and SEQ ID NO;

118, or a sequence complementary thereto, wherein the oligonucleotide is attached to a solid support.

7. The nucleic acid of claim 6, wherein the solid support is a microparticle.

8. The nucleic acid of claim 6, wherein the oligonucleotide has the sequence set forth in SEQ ID NO:

104 or the sequence complementary thereto.

9. The nucleic acid of claim 6, wherein the oligonucleotide has the sequence set forth in SEQ ID NO:

105 or a sequence complementary thereto.

10. The nucleic acid of claim 6, wherein the oligonucleotide has the sequence set forth in SEQ ID NO:

107 or a sequence complementary thereto.

11. The nucleic acid of claim 6, wherein the oligonucleotide has the sequence set forth in SEQ ID NO:

110 or a sequence complementary thereto.

12. The nucleic acid of claim 6, wherein the oligonucleotide has the sequence set forth in SEQ ID NO:

111 or a sequence complementary thereto.

13. The nucleic acid of claim 6, wherein the oligonucleotide has the sequence set forth in SEQ ID NO:

112 or a sequence complementary thereto.

14. The nucleic acid of claim 6, wherein the oligonucleotide has the sequence set forth in SEQ ID NO:

113 or a sequence complementary thereto.

15. The nucleic acid of claim 6, wherein the oligonucleotide has the sequence set forth in SEQ ID NO:

115 or a sequence complementary thereto.

16. The nucleic acid of claim 6, wherein the oligonucleotide has the sequence set forth in SEQ ID NO:

117 or a sequence complementary thereto.

17. The nucleic acid of claim 6, wherein the oligonucleotide has the sequence set forth in SEQ ID NO:

118 or a sequence complementary thereto.

18. An improved method of analyzing a biological sample suspected of comprising a target, wherein the improvement comprises using the tag or tag complement of claim 6.

19. A kit for sorting and identifying polynucleotides, the kit comprising at least one oligonucleotide tag or tag complement of claim 6.

20. The kit of claim 19, wherein each different oligonucleotide is linked to a defined location on the solid support, the defined location for each said oligonucleotide being different and distinguishable from the defined location for each different oligonucleotide.

21. The kit of claim 20, wherein the solid support is a microparticle.

22. The kit of claim 21, wherein each different oligonucleotide is linked to a different microparticle, each microparticle being distinguishable from each other microparticle.

23. A combination of oligonucleotide tags or tag complements for use in a multiplexed assay comprising at least one oligonucleotide set forth in SEQ ID NOs:

104, 105, 107, 110-113, 115, 117, and 118 and an oligonucleotide selected from the group consisting of SEQ ID NOs;

1-210, wherein the combination comprises at least two different oligonucleotides set forth in SEQ ID NOs;

1-210.

24. The combination of claim 23, wherein the oligonucleotides are linked to a solid support.

25. The combination of claim 24, wherein each different oligonucleotide is linked to a defined location on the solid support, the defined location for each said oligonucleotide being different than and distinguishable from the defined location for each different oligonucleotide.

26. The combination of claim 24, wherein the solid support is a microparticle.

27. The combination of claim 26, wherein each different oligonucleotide is linked to a different microparticle, each microparticle being distinguishable from each other microparticle.

28. A combination comprising a plurality of oligonucleotide tags or tag complements for use in a multiplexed assay, wherein the plurality of tags or tag complements comprise SEQ ID NOs:

104, 105, 107, 110-113, 115, 117, and 118 and sequences complementary thereto.