×

Polymerase enhancing factor (PEF) extracts, PEF protein complexes, isolated PEF protein, and methods for purifying and identifying

  • US 7,943,358 B2
  • Filed: 12/04/2006
  • Issued: 05/17/2011
  • Est. Priority Date: 03/21/1997
  • Status: Expired due to Fees
First Claim
Patent Images

1. A composition comprising:

  • (a) Pyrococcus furiosus DNA polymerase,(b) Thermus aquaticus DNA polymerase, and(c) a factor that at least partially inhibits the incorporation of dUTP or analogs thereof into a DNA polymer;

    wherein the factor is;

    a protein having dUTPase activity and encoded by a nucleic acid having the nucleotide sequence of SEQ ID NO;

    70 or a nucleic acid that hybridizes to the complete complement of the nucleic acid having the nucleotide sequence of SEQ ID NO;

    70, wherein the hybridization conditions comprise incubation in 5×

    SSC and 50% formamide at 42°

    C., and washing in 0.1×

    SSC and 0.1% sodium dodecyl sulfate at 60°

    C.;

    ora protein having a sequence of amino acids comprising the amino acid sequence of SEQ ID NO;

    71.

View all claims
  • 2 Assignments
Timeline View
Assignment View
    ×
    ×