Methods for expression and purification of pegylated recombinant human growth hormone containing a non-naturally encoded keto amino acid
First Claim
Patent Images
1. A method for producing an hGH-PEG conjugate comprising the steps:
- (a) reacting hGH comprising a non-naturally encoded keto amino acid with a derivatized polyethylene glycol (PEG) under conditions sufficient to covalently bond the keto amino acid to the PEG and to form the hGH-PEG conjugate wherein the non-naturally encoded keto amino acid is incorporated at position 35 in the hGH; and
(b) isolating and purifying said hGH-PEG conjugate by contacting said hGH-PEG conjugate with an anion exchange matrix under conditions that allow binding of the hGH-PEG conjugate to the matrix followed by elution and collection of the hGH-PEG conjugate from the anion exchange chromatography matrix to provide substantially purified hGH-PEG conjugate.
1 Assignment
0 Petitions
Accused Products
Abstract
The present invention relates generally to the production, purification, and isolation of human growth hormone (hGH). More particularly, the invention relates to the production, purification, and isolation of substantially purified hGH comprising non-naturally encoded amino acids and hGH from recombinant host cells or culture medium including, for example, yeast, insect, mammalian and bacterial host cells. The process of the present invention is also useful for purification of hGH linked to polymers or other molecules.
-
Citations
4 Claims
-
1. A method for producing an hGH-PEG conjugate comprising the steps:
- (a) reacting hGH comprising a non-naturally encoded keto amino acid with a derivatized polyethylene glycol (PEG) under conditions sufficient to covalently bond the keto amino acid to the PEG and to form the hGH-PEG conjugate wherein the non-naturally encoded keto amino acid is incorporated at position 35 in the hGH; and
(b) isolating and purifying said hGH-PEG conjugate by contacting said hGH-PEG conjugate with an anion exchange matrix under conditions that allow binding of the hGH-PEG conjugate to the matrix followed by elution and collection of the hGH-PEG conjugate from the anion exchange chromatography matrix to provide substantially purified hGH-PEG conjugate.
- (a) reacting hGH comprising a non-naturally encoded keto amino acid with a derivatized polyethylene glycol (PEG) under conditions sufficient to covalently bond the keto amino acid to the PEG and to form the hGH-PEG conjugate wherein the non-naturally encoded keto amino acid is incorporated at position 35 in the hGH; and
-
2. A method for isolating a substantially purified hGH-PEG conjugate comprising the steps of:
- a) culturing recombinant host cells capable of producing hGH comprising a non-naturally encoded keto amino acid in a liquid nutrient medium containing the non-naturally encoded amino acid under conditions which favor growth wherein the non-naturally encoded keto amino acid is incorporated at position 35 in the hGH;
b) inducing production of hGH comprising the non-naturally encoded keto amino acid by said cells;
c) contacting the hGH comprising a non-naturally encoded keto amino acid from step (b) with an anion exchange chromatography matrix under conditions that allow binding of the hGH to the matrix followed by eluting and collecting the hGH from the matrix;
d) contacting the hGH eluted from the anion exchange chromatography of step c) with a hydroxyapatite chromatography matrix under conditions that allow binding of the hGH to the matrix followed by eluting and collecting the hGH from the hydroxyapatite chromatography matrix;
e) contacting the hGH from step (d) with a hydrophobic interaction chromatography (HIC) matrix under conditions that allow binding of the hGH to the matrix followed by eluting and collecting the hGH from the HIC matrix to provide an hGH comprising a non-naturally encoded keto amino acid;
f) reacting the hGH comprising a non-naturally encoded amino acid from step (e) with a derivatized polyethylene glycol (PEG) under conditions sufficient to covalently bond the keto amino acid to the PEG and to form an hGH-PEG conjugate; and
g) isolating and purifying said hGH-PEG conjugate by contacting said hGH-PEG conjugate with an anion exchange matrix under conditions that allow binding of the hGH to the matrix followed by elution and collection of the hGH from the anion exchange chromatography matrix to provide substantially purified hGH-PEG conjugate.
- a) culturing recombinant host cells capable of producing hGH comprising a non-naturally encoded keto amino acid in a liquid nutrient medium containing the non-naturally encoded amino acid under conditions which favor growth wherein the non-naturally encoded keto amino acid is incorporated at position 35 in the hGH;
-
3. A method for isolating substantially purified hGH-PEG conjugates comprising the steps of:
- a) culturing recombinant host cells capable of producing hGH comprising a non-naturally encoded keto amino acid in a liquid nutrient medium containing the non-naturally encoded keto amino acid under conditions which favor growth wherein the non-naturally encoded keto amino acid is incorporated at position 35 in the hGH;
b) inducing production of hGH comprising the non-naturally encoded keto amino acid by said cells;
c) contacting hGH comprising a non-naturally encoded keto amino acid from step b) with an anion exchange chromatography matrix under conditions that allow binding of the hGH to the matrix followed by eluting and collecting the hGH from the matrix;
d) contacting the hGH from step c) with a hydrophobic interaction chromatography (HIC) matrix under conditions that allow binding of the hGH to the matrix followed by eluting and collecting the hGH from the HIC matrix to provide hGH comprising a non-naturally encoded keto amino acid;
e) reacting hGH comprising a non-naturally encoded keto amino acid from step d) with a derivatized polyethylene glycol (PEG) under conditions sufficient to covalently bond the keto amino acid to the PEG and to form an hGH-PEG conjugate; and
f) isolating and purifying said hGH-PEG conjugate by contacting said hGH-PEG conjugate with an anion exchange matrix under conditions that allow binding of the hGH to the matrix followed by elution and collection of the hGH from the anion exchange chromatography matrix to provide substantially purified hGH-PEG conjugate.
- a) culturing recombinant host cells capable of producing hGH comprising a non-naturally encoded keto amino acid in a liquid nutrient medium containing the non-naturally encoded keto amino acid under conditions which favor growth wherein the non-naturally encoded keto amino acid is incorporated at position 35 in the hGH;
-
4. A method for producing hGH-PEG conjugates comprising:
- a) culturing recombinant host cells capable of producing hGH comprising a non-naturally encoded keto amino acid in a liquid nutrient medium containing the non-naturally encoded keto amino acid under conditions which favor growth wherein the non-naturally encoded keto amino acid is incorporated at position 35 in the hGH;
b) inducing production of hGH comprising the non-naturally encoded keto amino acid by said cells;
c) reacting hGH comprising a non-naturally encoded keto amino acid from step b) with a derivatized polyethylene glycol (PEG) under conditions sufficient to covalently bond the keto amino acid to the PEG and to form an hGH-PEG conjugate and d) purifying said hGH-PEG conjugate with one or more of a hydroxyapatite chromatography matrix or hydrophobic interaction chromatography matrix.
- a) culturing recombinant host cells capable of producing hGH comprising a non-naturally encoded keto amino acid in a liquid nutrient medium containing the non-naturally encoded keto amino acid under conditions which favor growth wherein the non-naturally encoded keto amino acid is incorporated at position 35 in the hGH;
Specification