Nucleic acid encoding cytokine receptor ZCYTOR17

• US 7,947,474 B2
• Filed: 10/20/2006
• Issued: 05/24/2011
• Est. Priority Date: 06/26/2000
• Status: Active Grant

• Current Assignee: Zymogenetics Incorporated (Bristol-Myers Squibb Company)
• Original Assignee: Zymogenetics Incorporated (Bristol-Myers Squibb Company)
• Inventors: Maurer, Mark F., Kuijper, Joseph L., Gao, Zeren, Presnell, Scott R., Sprecher, Cindy A., Whitmore, Theodore E.
• Primary Examiner(s): Bunner; Bridget E
• Assistant Examiner(s): Hamud; Fozia M
• Application Number:
  US11/551,322
• Publication Number:
  US 20070048307A1
• Time in Patent Office: 1,677 Days
• Field of Search: None
• US Class Current: 435/69.52
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: C07K 14/715 for cytokines; for lymphokines; for interferons

Abstract

Novel polypeptides, polynucleotides encoding the polypeptides, and related compositions and methods are disclosed for zyctor17, a novel cytokine receptor. The polypeptides may be used within methods for detecting ligands that stimulate the proliferation and/or development of hematopoietic, lymphoid and myeloid cells in vitro and in vivo. Ligand-binding receptor polypeptides can also be used to block ligand activity in vitro and in vivo. The polynucleotides encoding zyctor17, are located on chromosome 5, and can be used to identify a region of the genome associated with human disease states. The present invention also includes methods for producing the protein, uses therefor and antibodies thereto.

Claims

1. An isolated polynucleotide encoding a polypeptide wherein the encoded polypeptide comprises an amino acid sequence having at least 95% sequence identity with amino acid residues 20-732 of SEQ ID NO:

2, wherein the encoded polypeptide stimulates inflammation upon binding zcytor17 ligand.

2. The isolated polynucleotide of claim 1 wherein the encoded polypeptide comprises amino acid residues 20-732 of SEQ ID NO:

2.

3. The isolated polynucleotide of claim 1 wherein the polynucleotide comprises nucleotides 228-2366 of SEQ ID NO:

1.

4. An isolated polynucleotide encoding a soluble receptor polypeptide wherein the encoded soluble receptor polypeptide comprises an amino acid sequence having at least 95% equence identity with amino acid residues 20-519 of SEQ ID NO:

2, wherein the encoded soluble receptor polypeptide reduces zcytor17 ligand induced inflammation.

5. The isolated polynucleotide of claim 4 wherein the encoded soluble receptor polypeptide comprises amino acid residues 20-519 of SEQ ID NO:

2.

6. The isolated polynucleotide of claim 4 wherein the polynucleotide comprises nucleotides 228-1727 of SEQ ID NO:

1.

7. An expression vector comprising the following operably linked elements:

a transcription promoter;

a DNA segment encoding a polypeptide comprising an amino acid sequence having at least 95% sequence identity with amino acid residues 20-732 of SEQ ID NO;

2, wherein the encoded polypeptide stimulates inflammation upon binding zcytor17 ligand; and

a transcription terminator; and

wherein the promoter is operably linked to the DNA segment, and the DNA segment is operably linked to the transcription terminator.

8. The expression vector of claim 7 further comprising a secretory signal sequence operably linked to the DNA segment.

9. A cultured cell comprising the expression vector of claim 7, wherein the cell expresses the polypeptide encoded by the DNA segment.

13. A method of producing a polypeptide comprising:

culturing a cell according to claim 9; and

isolating the polypeptide produced by the cell.

10. An expression vector comprising the following operably linked elements:

a transcription promoter;

a DNA segment encoding a soluble receptor polypeptide comprising an amino acid sequence having at least 95% sequence identity with amino acid residues 20-519 of SEQ ID NO;

2, wherein the encoded soluble receptor polypeptide reduces zcytor17 ligand induced inflammation; and

a transcription terminator; and

wherein the promoter is operably linked to the DNA segment, and the DNA segment is operably linked to the transcription terminator.

11. The expression vector of claim 10 further comprising a secretory signal sequence operably linked to the DNA segment.

12. A cultured cell comprising the expression vector of claim 10, wherein the cell expresses the polypeptide encoded by the DNA segment.

14. A method of producing a polypeptide comprising:

culturing a cell according to claim 12; and

isolating the polypeptide produced by the cell.

15. A DNA construct encoding a fusion protein, the DNA construct comprising:

a first DNA segment encoding a soluble receptor polypeptide comprising an amino acid sequence having at least 95% sequence identity with amino acid residues 20-519 of SEQ ID NO;

2;

a second DNA segment encoding an immunoglobulin heavy chain constant region;

wherein the first and second DNA segments are connected in-frame;

wherein the first and second DNA segments encode the fusion protein; and

wherein fusion protein reduces zcytor17 ligand induced inflammation.

16. The DNA construct of claim 15 wherein the soluble receptor polypeptide comprises amino acid residues 20-519 of SEQ ID NO:

2.

17. The DNA construct of claim 15 wherein the immunoglobulin heavy chain constant region is a human immunoglobulin F_c4 fragment.