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Emulsion PCR and amplicon capture

  • US 7,947,477 B2
  • Filed: 01/25/2010
  • Issued: 05/24/2011
  • Est. Priority Date: 06/30/2006
  • Status: Active Grant
First Claim
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1. A method of attaching a polynucleotide to a surface comprising:

  • generating a hydrophilic compartment of an inverse emulsion comprising a polynucleotide and a surface,wherein said-polynucleotide comprises an isoC or isoG to which a photoactivatable binding moiety and said surface comprises a binding moiety binding partner, andactivating said binding moiety by exposing said compartment to activating light, which activated binding moiety binds to said binding partner, whereby said polynucleotide is attached to said surface.

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