Method of dynamically culturing embryonic stem cells
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1. A method of dynamically generating embryoid bodies comprising culturing embryonic stem cells in slow turning lateral vessel bioreactor which subjects cells cultured in a fluid contained therein to randomized gravity vectors without formation of gas bubbles in said fluid to thereby obtain dynamically generated embryoid bodies.
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Abstract
The present invention is of a method of dynamically generating human embryoid bodies which can be used for generating lineage specific cells and cell lines. Specifically, the present invention can be used to generate ESC-differentiated cells for cell-replacement therapy.
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13 Claims
- 1. A method of dynamically generating embryoid bodies comprising culturing embryonic stem cells in slow turning lateral vessel bioreactor which subjects cells cultured in a fluid contained therein to randomized gravity vectors without formation of gas bubbles in said fluid to thereby obtain dynamically generated embryoid bodies.
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