Imaging apparatus with selectable moveable stage

• US 7,949,383 B2
• Filed: 05/27/2005
• Issued: 05/24/2011
• Est. Priority Date: 02/25/2000
• Status: Active Grant

• Current Assignee: Xenogen Corporation (Revvity, Inc.)
• Original Assignee: Xenogen Corporation (Revvity, Inc.)
• Inventors: Nelson, Michael Bo, Sprague, David N, Rice, Bradley W., Cable, Michael D.
• Primary Examiner(s): Le; Long V
• Assistant Examiner(s): BOR, HELENE CATHERINE
• Application Number:
  US11/139,256
• Publication Number:
  US 20050231592A1
• Time in Patent Office: 2,188 Days
• Field of Search: 600/473, 600/476, 359/398, 359/391, 359/393
• US Class Current: 600/407
• CPC Class Codes: G01N 21/01 Arrangements or apparatus for facilitating the optical investigation
• CPC Class Codes: G02B 21/16 adapted for ultraviolet illumination ; Fluorescence microscopes G02B21/0076 takes precedence
• CPC Class Codes: G02B 27/0018 with means for preventing ghost images anti-reflection coatings G02B1/11

Abstract

An imaging box assembly is provided for capturing an image of a sample. The imaging box assembly includes a body having an interior cavity for receiving the sample, and having a front portion defining an opening into the cavity. The body further includes a view port enabling viewing of the sample contained in the interior cavity. A door is mounted to the body that is movable between an opened condition, enabling access to the interior cavity through the cavity opening, and a closed condition, positioning a door rear portion substantially adjacent the body front portion to prevent access through the cavity opening. The box assembly further includes a moveable stage disposed in the cavity interior that supports the sample. The moveable stage is adapted to selectively position the sample at a selected one of a plurality of positions relative to the view hole.

Claims

1. A light-tight imaging system for capturing an image of a sample, the imaging system comprising:

an imaging box including;

a body including an interior cavity for receiving the sample, and having a front portion defining an opening into said cavity, said body further including a view hole enabling viewing of the sample; and

a door having a rear portion, said door movable between an opened condition, enabling access to the interior cavity through the cavity opening, and a closed condition, positioning said rear portion substantially adjacent the body front portion to prevent access through the cavity opening;

a moveable stage disposed in the cavity interior that supports the sample, said moveable stage being adapted to selectively position the sample at a selected one of a plurality of vertical positions relative to the view hole, wherein each of the plurality of vertical positions has substantially the same horizontal position in the interior cavity and each is substantially linearly closer to or substantially linearly further away from said view hole with respect to every other vertical position; and

a light shield supported by said moveable stage, said light shield preventing light from said sample to translate horizontally to a portion of the moveable stage that does not support said sample, wherein said light shield includes a substantially vertical wall upstanding from a surface of the moveable stage.

2. The imaging system according to claim 1, further including:

a temperature control element configured to alter the temperature of one of the sample and the interior cavity.

3. The imaging system according to claim 2, wherein the temperature control element includes a thermal sheet in thermal communication with said moveable stage.

4. The imaging system according to claim 1, further including:

a gas manifold device in communication with said interior cavity, and configured to supply a gas to the sample, wherein said gas manifold device is coupled to said moveable stage.

5. The imaging system according to claim 1, further including:

a positioning sensor, wherein said positioning sensor cooperates with the moveable stage to determine its position relative to said view port.

6. The imaging system according to claim 5, wherein said positioning sensor includes a laser positioned in said interior cavity to intercept said moveable stage at the selected one of a plurality of positions relative to the view hole at said first vertical position.

7. The imaging system according to claim 1, further including:

a drive assembly coupled to the moveable stage to selectively move said moveable stage to the selected one of a plurality of positions.

8. The imaging system according to claim 1, further including:

a partition located in the cavity interior, the partition forming a compartment within the body separate from the interior cavity.

9. An imaging system for capturing an image of a sample, the imaging system comprising:

an imaging box having;

a body including an interior cavity for receiving the sample, and having a front wall defining an opening into said cavity;

a door having a rear wall and an exterior face, said door movable between an opened condition, enabling access to the interior cavity through the cavity opening, and a closed condition, positioning said rear wall substantially adjacent the body front wall to prevent access through the cavity opening;

a moveable stage in the cavity interior that supports the sample, the moveable stage having a first vertical position and a second vertical position in the interior cavity, wherein the first vertical position and the second vertical position have the substantially same horizontal position in the interior cavity, and

a light shield supported by said moveable stage, said light shield preventing light from said sample to translate horizontally to a portion of the moveable stage that does not support said sample, wherein said light shield includes a substantially vertical wall upstanding from a surface of the moveable stage.

10. The imaging system of claim 9 further including:

a temperature control element configured to alter the temperature of one of the sample or the interior cavity, wherein the temperature control element is a thermal sheet in thermal communication with said moveable stage.

11. The imaging system of claim 9, further including:

a gas manifold coupled to said moveable stage, the gas manifold configured to supply a gas to the sample.

12. The imaging system of claim 9, further including:

a laser positioned in said interior cavity to intercept said moveable stage at said first vertical position.

13. The imaging system of claim 9, further including:

a worm gear coupled to the moveable stage that provides vertical translation of the moveable stage between the first vertical position and the second vertical position.

14. The imaging system of claim 9, further including:

a partition located in the cavity interior, the partition forming a compartment within the body separate from the interior cavity.

15. The imaging system of claim 14, wherein the compartment includes an opening formed in the body and the compartment is adapted to slideably receive a drawer through the opening.

16. The imaging system of claim 15, wherein the drawer houses electronics equipment used to control components within the imaging box.