Methods, devices, kits and compositions for detecting roundworm, whipworm, and hookworm
First Claim
1. A method of detecting the presence or absence of one or more helminthic antigens in a sample, the method comprising:
- (a) contacting a sample from a mammal with at least two antibodies selected from the group consisting of;
(i) a first antibody capable of specifically binding a roundworm coproantigen, but not a whipworm or hookworm coproantigen;
(ii) a second antibody capable of specifically binding a whipworm coproantigen, but not a roundworm or hookworm coproantigen; and
(iii) a third antibody capable of specifically binding a hookworm coproantigen, but not a whipworm or roundworm coproantigen;
(b) forming antibody-coproantigen complexes in the presence of the coproantigens, if any, in the sample; and
(c) detecting the presence or absence of the antibody-coproantigen complexes, if any.
3 Assignments
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Accused Products
Abstract
Methods, devices, kits and compositions for detecting the presence or absence of one or more helminthic coproantigens in a sample are disclosed herein. The methods, devices, kits and compositions of the present invention may be used to confirm the presence or absence of roundworm, whipworm and/or hookworm in a fecal sample from a mammal and may also be able to distinguish between one or more helminth infections. Confirmation of the presence or absence of roundworm, whipworm and/or hookworm in the mammal may be made, for example, for the purpose of selecting an optimal course of treating the mammal and/or for the purpose of determining whether the mammal has been rid of the infection after treatment has been initiated.
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Citations
39 Claims
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1. A method of detecting the presence or absence of one or more helminthic antigens in a sample, the method comprising:
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(a) contacting a sample from a mammal with at least two antibodies selected from the group consisting of; (i) a first antibody capable of specifically binding a roundworm coproantigen, but not a whipworm or hookworm coproantigen; (ii) a second antibody capable of specifically binding a whipworm coproantigen, but not a roundworm or hookworm coproantigen; and (iii) a third antibody capable of specifically binding a hookworm coproantigen, but not a whipworm or roundworm coproantigen; (b) forming antibody-coproantigen complexes in the presence of the coproantigens, if any, in the sample; and (c) detecting the presence or absence of the antibody-coproantigen complexes, if any. - View Dependent Claims (2, 3, 4, 5, 6, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25)
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7. A method of diagnosing whether a mammal is infected with one or more parasitic worms, the method comprising the steps of:
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(a) contacting a sample from a mammal with at least two antibodies selected from the group consisting of; (i) a first antibody capable of specifically binding a roundworm coproantigen, but not a whipworm or hookworm coproantigen; (ii) a second antibody capable of specifically binding a whipworm coproantigen, but not a roundworm or hookworm coproantigen; and (iii) a third antibody capable of specifically binding a hookworm coproantigen, but not a whipworm or roundworm coproantigen; (b) forming antibody-coproantigen complexes in the presence of the coproantigens, if any, in the sample; (c) detecting the presence or absence of the antibody-coproantigen complexes, if any; and (d) diagnosing the mammal as having; (i) a roundworm infection if a roundworm antibody-coproantigen complex is present; (ii) a whipworm infection if a whipworm antibody-coproantigen complex is present; and (iii) a hookworm infection if a hookworm antibody-coproantigen complex is present. - View Dependent Claims (8, 9, 10, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39)
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Specification