Liquid crystals with reduced toxicity and applications thereof

• US 7,951,577 B2
• Filed: 10/23/2007
• Issued: 05/31/2011
• Est. Priority Date: 07/17/2003
• Status: Expired due to Fees

• Current Assignee: Wisconsin Alumni Research Foundation (University of Wisconsin System)
• Original Assignee: Wisconsin Alumni Research Foundation (University of Wisconsin System)
• Inventors: Jang, Chang-Hyun, Murphy, Christopher John, Campbell, Sean Francis, Cheng, Li-Lin, Luk, Yan-Yeung, Abbott, Nicholas L.
• Primary Examiner(s): WU, SHEAN CHIU
• Application Number:
  US11/877,029
• Publication Number:
  US 20080050799A1
• Time in Patent Office: 1,316 Days
• Field of Search: 252/299.01, 252/299.63, 252/299.5, 252/299.7, 435/243, 435/404
• US Class Current: 435/243
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C07D 311/24 with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached in position 2
• CPC Class Codes: C09K 19/3003 Compounds containing at least two rings in which the different rings are directly linked (covalent bond)
• CPC Class Codes: C09K 19/42 Mixtures of liquid crystal compounds covered by two or more of the preceding groups C09K19/06 - C09K19/40
• CPC Class Codes: C09K 2019/0407 containing a carbocyclic ring, e.g. dicyano-benzene, chlorofluoro-benzene or cyclohexanone
• CPC Class Codes: C09K 2019/301 Cy-Cy-Ph

Abstract

Liquid crystal compositions that exhibit little or no toxicity with respect to cells include liquid crystals with chemical functional groups such as fluorine atoms, fluorophenyl groups, or difluorophenyl groups. Liquid crystals with little or no toxicity to cell lines may be added to cell culture media or added to components used in cell culture media. Cells may be grown in cell culture media that includes liquid crystals that exhibit little or no toxicity to cells.

Claims

1. A liquid crystal cell culture media, comprising at least one liquid crystal compound and at least one cell culture component selected from a vitamin, an amino acid, a sugar, or combinations thereof, wherein the at least one liquid crystal comprises a compound comprising a fluorinated phenyl group, a fluorinated cyclohexyl group;

a cholesteric liquid crystal, or a liquid crystal that exhibits reduced toxicity towards cells.

2. The liquid crystal cell culture media of claim 1, wherein the cell culture media comprises at least three amino acids selected from L-alanine, L-arginine, L-asparagine, L-aspartic acid, L-cystine, L-glutamic acid, L-glutamine, glycine, L-histidine, hydroxy-L-proline, L-isoleucine, L-leucine, L-lysine, L-methionine, L-ornithine, L-phenylalanine, L-proline, L-serine, L-taurine, L-threonine, L-tryptophan, L-tyrosine, L-valine, or salts or combinations thereof.

3. The liquid crystal cell culture media of claim 1, wherein the cell culture media comprises at least five amino acids selected from L-alanine, L-arginine, L-asparagine, L-aspartic acid, L-cystine, L-glutamic acid, L-glutamine, glycine, L-histidine, hydroxy-L-proline, L-isoleucine, L-leucine, L-lysine, L-methionine, L-ornithine, L-phenylalanine, L-proline, L-serine, L-taurine, L-threonine, L-tryptophan, L-tyrosine, L-valine, or salts or combinations thereof.

4. The liquid crystal cell culture media of claim 1, wherein the cell culture media comprises at least eight amino acids selected from L-alanine, L-arginine, L-asparagine, L-aspartic acid, L-cystine, L-glutamic acid, L-glutamine, glycine, L-histidine, hydroxy-L-proline, L-isoleucine, L-leucine, L-lysine, L-methionine, L-ornithine, L-phenylalanine, L-proline, L-serine, L-taurine, L-threonine, L-tryptophan, L-tyrosine, L-valine, or salts or combinations thereof.

5. The liquid crystal cell culture media of claim 1, wherein the cell culture media further comprises at least two salts selected from CaCl₂, NaCl, NaHCO₃, Na₂HPO₄, NaH₂PO₄, KCl, K₂HPO₄, KH₂PO₄, MgSO₄, MgCl₂, Fe(NO₃)₃, FeSO₄, CuSO₄, ZnSO₄, ZnCl₂, CoCl₂, CuCl₂, MnCl₂, (NH₄)₂MoO₄, or hydrates or combinations thereof.

6. The liquid crystal cell culture media of claim 1, wherein the cell culture media comprises at least two vitamins selected from ascorbic acid, D-biotin, choline, choline chloride, choline bitartrate, folic acid, myo-inositol, inositol, niacin, niacinamide, nicotinamide, p-aminobenzoic acid, D-pantothenic acid, pyridoxine, pyridoxal, riboflavin, DL-thioctic acid, thiamine, vitamin B12, vitamin A alcohol, vitamin D-2, vitamin E, menadione, nicotinic acid, alpha-tocopherol, or salts or combinations thereof.

7. The liquid crystal cell culture media of claim 1, wherein the cell culture media comprises at least five vitamins selected from ascorbic acid, D-biotin, choline, choline chloride, choline bitartrate, folic acid, myo-inositol, inositol, niacin, niacinamide, nicotinamide, p-aminobenzoic acid, D-pantothenic acid, pyridoxine, pyridoxal, riboflavin, DL-thioctic acid, thiamine, vitamin B12, vitamin A alcohol, vitamin D-2, vitamin E, menadione, nicotinic acid, alpha-tocopherol, or salts or combinations thereof.

8. The liquid crystal cell culture media of claim 1, wherein the cell culture media comprises at least eight vitamins selected from ascorbic acid, D-biotin, choline, choline chloride, choline bitartrate, folic acid, myo-inositol, inositol, niacin, niacinamide, nicotinamide, p-aminobenzoic acid, D-pantothenic acid, pyridoxine, pyridoxal, riboflavin, DL-thioctic acid, thiamine, vitamin B12, vitamin A alcohol, vitamin D-2, vitamin E, menadione, nicotinic acid, alpha-tocopherol, or salts or combinations thereof.

9. The liquid crystal cell culture media of claim 1, wherein the at least one liquid crystal is a cholesteric liquid crystal.

10. The liquid crystal cell culture media of claim 1, wherein the at least one liquid crystal comprises a fluorinated cyclohexyl group.

11. The liquid crystal cell culture media of claim 1, wherein the at least one liquid crystal comprises a fluorinated phenyl group.

12. The liquid crystal cell culture media of claim 1, wherein the at least one liquid crystal comprises a difluorinated phenyl group.

13. The liquid crystal cell culture media of claim 1, wherein the at least one liquid crystal comprises a difluorinated compound having the following formula where Z is an alkyl group having 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, or 16 carbon atoms

14. The liquid crystal cell culture media of claim 1, wherein the at least one liquid crystal exhibits lyotropic or a thermotropic properties.

15. The liquid crystal cell culture media of claim 1, wherein the liquid crystal cell culture media comprises 2 or more different liquid crystals.

16. The liquid crystal cell culture media of claim 1, further comprising water.

17. The liquid crystal cell culture media of claim 1, wherein the at least one cell culture component comprises glucose.