Method and reagent system with non-regenerable enzyme-coenzyme complex
First Claim
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1. An apparatus for detecting an analyte in a sample, the analyte being present in the sample only up to a predetermined maximum possible concentration, the apparatus comprising:
- (a) a support configured to receive a first volume of the sample; and
(b) a detection reagent provided on the support, the detection reagent comprising an enzyme-coenzyme complex, the detection reagent being provided in an amount such that the amount of enzyme-coenzyme complex is at least stoichiometric relative to the amount of analyte present in the sample;
wherein the enzyme-coenzyme complex is selected and configured to participate in a reaction with the analyte, and wherein the detection reagent does not include any mediator capable of reacting with the coenzyme.
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Abstract
The present invention is generally directed towards a reagent system for detecting an analyte in a sample. The reagent system has a detection reagent comprising an enzyme-coenzyme complex in a form such that no regeneration of the coenzyme takes place, whereby the enzyme-coenzyme complex is employed in an at least stoichiometric amount relative to the analyte present in the sample, and a support to receive the detection reagent.
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Citations
12 Claims
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1. An apparatus for detecting an analyte in a sample, the analyte being present in the sample only up to a predetermined maximum possible concentration, the apparatus comprising:
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(a) a support configured to receive a first volume of the sample; and (b) a detection reagent provided on the support, the detection reagent comprising an enzyme-coenzyme complex, the detection reagent being provided in an amount such that the amount of enzyme-coenzyme complex is at least stoichiometric relative to the amount of analyte present in the sample; wherein the enzyme-coenzyme complex is selected and configured to participate in a reaction with the analyte, and wherein the detection reagent does not include any mediator capable of reacting with the coenzyme. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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Specification