Culture medium for gingival fibroblasts
First Claim
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1. A method for the culture of gingival fibroblasts which comprises growing gingival fibroblasts in a gingival fibroblast culture medium, wherein said medium comprises a culture medium for animal or human cells, free of animal serum to which is added:
- from 0.1 ng/ml to 100 ng/ml bFGF and from 1 μ
g/ml to 50 μ
g/ml insulin.
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Abstract
The present invention relates to a gingival fibroblast culture medium free of animal serum, comprising an animal cell culture medium, free of animal serum, to which is added:
- from 0.1 ng/ml to 100 ng/ml bFGF, and/or
- from 1 μg/ml to 50 μg/ml insulin.
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Citations
6 Claims
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1. A method for the culture of gingival fibroblasts which comprises growing gingival fibroblasts in a gingival fibroblast culture medium, wherein said medium comprises a culture medium for animal or human cells, free of animal serum to which is added:
- from 0.1 ng/ml to 100 ng/ml bFGF and from 1 μ
g/ml to 50 μ
g/ml insulin. - View Dependent Claims (3, 4, 5, 6)
- from 0.1 ng/ml to 100 ng/ml bFGF and from 1 μ
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2. A kit for cultivating gingival fibroblasts, comprising:
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a gingival fibroblast culture medium comprising a culture medium for animal or human cells, free of animal serum to which is added;
from 0.1 ng/ml to 100 ng/ml bFGF and from 1 μ
g/ml to 50 μ
g/ml insulin andplatelet lysate.
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Specification
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Current AssigneeInserm Transfert SA (Allianz Global Investors GmbH), L'Assistance Publique - Hopitaux De Paris, Paris Descartes University, Universite Paris CITE
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Original AssigneeUniversité Paris Descartes
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InventorsCoulomb, Bernard, Lafont, Antoine, Gogly, Bruno
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Primary Examiner(s)Romeo; David S
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Assistant Examiner(s)Gamett; Daniel C
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Application NumberUS12/051,559Publication NumberTime in Patent Office1,168 DaysField of SearchNoneUS Class Current435/405CPC Class CodesC12N 2500/05 Inorganic componentsC12N 2500/20 Transition metalsC12N 2500/25 Insulin-transferrin; Insuli...C12N 2500/32 Amino acidsC12N 2500/84 from mammalsC12N 2500/90 Serum-free medium, which ma...C12N 2501/115 Basic fibroblast growth fac...C12N 5/0656 Adult fibroblasts
