Device and method for making discrete volumes of a first fluid in contact with a second fluid, which are immiscible with each other
First Claim
Patent Images
1. A method comprising:
- providing a first fluid disposed in a container having a bottom;
ejecting a volume of a second fluid from a through-hole in the container bottom, thereby forming a first droplet of the second fluid in the first fluid, which first droplet rises in the first fluid relative to an intake tip of a conduit such that the first droplet is disposed adjacent the intake tip, wherein the first fluid and the second fluid are immiscible with respect to one another and the second fluid is less dense than the first fluid;
moving at least one of the first droplet and an intake tip of a conduit relative to one another such that the first droplet is disposed adjacent the intake tip when ejected; and
drawing the first droplet and an amount of the first fluid through the intake tip and into the conduit.
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Abstract
Various embodiments described in the application relate to an apparatus, system, and method for generating, within a conduit, discrete volumes of one or more fluids that are immiscible with a second fluid. The discrete volumes can be used for biochemical or molecular biology procedures involving small volumes, for example, microliter-sized volumes, nanoliter-sized volumes, or smaller. The system can comprise an apparatus comprising at least one conduit operatively connected to one or more pumps for providing discrete volumes separated from one another by a fluid that is immiscible with the fluid(s) of the discrete volumes, for example, aqueous immiscible-fluid-discrete volumes separated by an oil.
174 Citations
10 Claims
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1. A method comprising:
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providing a first fluid disposed in a container having a bottom; ejecting a volume of a second fluid from a through-hole in the container bottom, thereby forming a first droplet of the second fluid in the first fluid, which first droplet rises in the first fluid relative to an intake tip of a conduit such that the first droplet is disposed adjacent the intake tip, wherein the first fluid and the second fluid are immiscible with respect to one another and the second fluid is less dense than the first fluid; moving at least one of the first droplet and an intake tip of a conduit relative to one another such that the first droplet is disposed adjacent the intake tip when ejected; and drawing the first droplet and an amount of the first fluid through the intake tip and into the conduit. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A method comprising:
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forming a first droplet of a second fluid on a film; submersing the film in a first fluid, wherein the first fluid and the second fluid are immiscible with respect to one another and the second fluid has a greater density than the first fluid; moving at least one of the first droplet and an intake tip of a conduit relative to one another such that the first droplet is disposed adjacent the intake tip when ejected; and drawing the first droplet and an amount of the first fluid through the intake tip and into the conduit. - View Dependent Claims (9, 10)
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Specification