Biologically active substance transfer sheet, cell culture kit constituted of cell culture plate and biologically active substance transfer sheet, producing method thereof and method for screening cell culture conditions utilizing the same

• US 7,960,168 B2
• Filed: 07/30/2008
• Issued: 06/14/2011
• Est. Priority Date: 12/16/2003
• Status: Expired due to Fees

• Current Assignee: Brad Jackson
• Original Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Inventors: Miyazaki, Takeshi, Fujiyama, Tomoyo, Kanome, Osamu, Matsuda, Ryoichi, Watanabe, Kohei
• Primary Examiner(s): Weber; Jon P
• Assistant Examiner(s): Shen; Bin
• Application Number:
  US12/183,008
• Publication Number:
  US 20080293138A1
• Time in Patent Office: 1,049 Days
• Field of Search: None
• US Class Current: 435/287.1
• CPC Class Codes: B01L 2300/0812 Bands; Tapes
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/123 Flexible; Elastomeric
• CPC Class Codes: B01L 3/0244 using pins
• CPC Class Codes: B01L 3/0262 using touch-off at substrate or container
• CPC Class Codes: B01L 3/0293 for liquids
• CPC Class Codes: B01L 3/50853 with covers or lids
• CPC Class Codes: C12M 23/12 Well or multiwell plates C12M25/04 takes precedence
• CPC Class Codes: C12M 23/38 Caps; Covers; Plugs; Pouring means
• CPC Class Codes: C12M 29/04 Filters; Permeable or porous membranes or plates, e.g. dialysis
• CPC Class Codes: C12Q 1/02 involving viable microorganisms
• CPC Class Codes: G01N 33/5005 involving human or animal cells immunoassay G01N33/56966; immunoassays of protozoa G01N33/56905; protozoa in screening assays C12Q1/025

Abstract

The invention provides a biologically active substance transfer sheet which can be formed with simple steps, a cell culture kit which comprises a cell culture plate and a biologically active substance transfer sheet, a producing method therefor, and a method for screening cell culture conditions with cells. The transfer sheet is prepared by providing biologically active substances having biological activity in plural areas on a sheet base, and biologically active substances are supplied from the sheet to culture regions provided on the plate.

Claims

1. A cell culture kit comprising:

a culture plate having two or more discrete culture regions for culturing a cell; and

a sheet having covering portions discretely arranged to cover the respective culture regions,

wherein each of the culture regions holds a first biologically active substance, and each of the covering portions holds a second biologically active substance, each of the first and second biologically active substances having a biological activity on a cell,

wherein the sheet is made from an elastic or flexible film at least at the covering portions,

wherein the first biologically active substance is immobilized on the culture regions, and the second biologically active substance is attached to the covering portions, and

wherein the sheet constitutes a transfer sheet for transferring the second biologically active substance to the culture regions.

2. The cell culture kit according to claim 1, wherein the culture regions hold the first biologically active substance in different concentrations.

3. The cell culture kit according to claim 1, wherein the each of the culture regions is formed in a recess formed in the plate.

4. The cell culture kit according to claim 1, wherein each of the culture regions is surrounded by a protruding wall-shaped structure.

5. The cell culture kit according to claim 1, wherein a layer for holding the second biologically active substance is formed on an entire or partial surface of the sheet.

6. The cell culture kit according to claim 5, wherein the holding layer is able to release the second biologically active substance during cell culturing or the holding layer is able to sustainably release the second biologically active substance.

7. The cell culture kit according to claim 1, wherein each of the covering portions is formed in a recess formed in the sheet.

8. The cell culture kit according to claim 1, wherein each of the covering portions is formed on a protruding portion formed on the sheet.

9. The cell culture kit according to claim 1, wherein each of the covering portions is surrounded by a protruding wall-shaped structure formed on the sheet.

10. The cell culture kit according to claim 1, wherein the second biologically active substance is attached to the covering portions so as to be released when it is in contact with a culture liquid.

11. The cell culture kit according to claim 1, wherein the second biologically active substance is attached to each of the covering portions by discharging a liquid droplet containing the second biologically active substance.

12. A method of culturing a cell with a cell culture kit as defined in any one of claims 1 and 2 to 9, comprising a step of covering the culture regions on the culture plate with the covering portions of the sheet and a step of contacting a cell with a biologically active substance in a culture liquid in the culture regions.