Polynucleotides for use as tags and tag complements, manufacture and use thereof
First Claim
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1. A composition comprising a plurality of chemically synthesized oligonucleotide tag complements, each oligonucleotide having a 5′
- residue and a 3′
residue and being characterized in that;
(a) each oligonucleotide is free of either cytosine or guanosine residues;
(b) any two cytosine or guanosine residues are separated by at most 6 non-cytosine or non-guanosine residues, respectively;
(c) the length of each oligonucleotide differs by no more than five bases from the average length of all oligonucleotides in the composition; and
(d) when each oligonucleotide tag complement is exposed to hybridization conditions comprising 0.2M NaCl, 0.1M Tris, 0.08% Triton X-100, pH 8.0 at 37°
C., the maximum degree of hybridization between the tag complement and a tag not fully complementary to the tag complement does not exceed 25% of the degree of hybridization between the tag complement and its fully complementary tag.
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Abstract
A family of minimally cross-hybridizing nucleotide sequences, methods of use, etc. A specific family of 210 24mers is described.
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20 Claims
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1. A composition comprising a plurality of chemically synthesized oligonucleotide tag complements, each oligonucleotide having a 5′
- residue and a 3′
residue and being characterized in that;(a) each oligonucleotide is free of either cytosine or guanosine residues; (b) any two cytosine or guanosine residues are separated by at most 6 non-cytosine or non-guanosine residues, respectively; (c) the length of each oligonucleotide differs by no more than five bases from the average length of all oligonucleotides in the composition; and (d) when each oligonucleotide tag complement is exposed to hybridization conditions comprising 0.2M NaCl, 0.1M Tris, 0.08% Triton X-100, pH 8.0 at 37°
C., the maximum degree of hybridization between the tag complement and a tag not fully complementary to the tag complement does not exceed 25% of the degree of hybridization between the tag complement and its fully complementary tag. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
- residue and a 3′
Specification