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One-step enzymatic and amine detection technique

  • US 7,964,340 B2
  • Filed: 04/21/2009
  • Issued: 06/21/2011
  • Est. Priority Date: 06/30/2004
  • Status: Expired due to Fees
First Claim
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1. A method for detecting an amine, enzyme, or enzyme inhibitor within a test sample, the method comprising:

  • i) contacting an incubation mixture that comprises the test sample that may contain an amine, enzyme, or enzyme inhibitor and further comprises a plurality of reactive complexes that each comprises a substrate joined to a reporter and a specific binding member, said substrate being cleavable by a hydrolytic enzyme to release said reporter with a chromatographic medium, said chromatographic medium defining an enzyme detection zone within which is immobilized a receptive material that has an affinity for said specific binding member for generating an enzyme detection signal wherein the presence or quantity of a hydrolytic enzyme is determinable from said enzyme detection signal, and an amine detection zone positioned downstream from said enzyme detection zone, wherein a chemichromic dye is contained within said amine detection zone, said chemichromic dye being capable of undergoing a color change in the presence of an amine, wherein the presence or quantity of an amine is determinable from said color change;

    ii) determining the presence or quantity of an enzyme or enzyme inhibitor from said enzyme detection signal; and

    iii) determining the presence or quantity of an amine from said amine detection signal.

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