Labels for electronic detection of individual molecules and methods for their detection
First Claim
1. A method for the detection of individual target analyte molecules in a sample comprising:
- (a) contacting one or more labels, each of which comprises a target specific probe capable of binding the target analyte molecule attached to a polymeric molecule or filament which comprises one or more elements with different electrical properties as detected by a nanoelectrode, with a target analyte molecule;
(b) moving label which comprises the target specific probe that is bound to the target analyte molecule sufficiently close to a nanoelectrode such that the electric current of the nanoelectrode is changed; and
(c) detecting the changes in electric current produced in the nanoelectrode by the elements with different electrical properties in label that is bound to the target analyte molecule, wherein the method does not include detecting changes in fluorescence.
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Abstract
The invention provides labels for electronic detection of individual molecules. The labels are comprised of elements with different electrical properties that affect the electric current flowing through a nanoelectrode. The labels are of polymeric or filamentous structure where the elements are arranged linearly along their length. The arrangement of the elements is predetermined and combinatorial, so that a high diversity of labels can be generated in a manner that resembles barcoding on a nanoscale level. Methods for the synthesis of said barcode labels and for the binding of the barcode labels to individual molecules, their movement past a nanoelectrode, and their detection are also provided.
7 Citations
18 Claims
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1. A method for the detection of individual target analyte molecules in a sample comprising:
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(a) contacting one or more labels, each of which comprises a target specific probe capable of binding the target analyte molecule attached to a polymeric molecule or filament which comprises one or more elements with different electrical properties as detected by a nanoelectrode, with a target analyte molecule; (b) moving label which comprises the target specific probe that is bound to the target analyte molecule sufficiently close to a nanoelectrode such that the electric current of the nanoelectrode is changed; and (c) detecting the changes in electric current produced in the nanoelectrode by the elements with different electrical properties in label that is bound to the target analyte molecule, wherein the method does not include detecting changes in fluorescence. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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Specification