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3′-OH unblocked nucleotides and nucleosides, base modified with labels and photocleavable, terminating groups and methods for their use in DNA sequencing

  • US 7,964,352 B2
  • Filed: 11/11/2008
  • Issued: 06/21/2011
  • Est. Priority Date: 12/05/2006
  • Status: Active Grant
First Claim
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1. A method of sequencing a target nucleic acid comprising the following steps:

  • (i) attaching the 5′

    -end of a primer to a solid surface;

    (ii) hybridizing a target nucleic acid to the primer attached to the solid surface to form a hybridized primer/target nucleic acid complex;

    (iii) obtaining a polymerase and one or more compounds according to formula I, II, III, IV, V, VI or VII;

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