Sensor system

• US 7,964,390 B2
• Filed: 02/03/2005
• Issued: 06/21/2011
• Est. Priority Date: 10/11/2002
• Status: Active Grant

• Current Assignee: Case Western Reserve University
• Original Assignee: Case Western Reserve University
• Inventors: Yang, Jian, Tohida, Koji, Rozakis, George, Gratzl, Miklos
• Primary Examiner(s): BOWERS, NATHAN ANDREW
• Application Number:
  US11/050,584
• Publication Number:
  US 20050221276A1
• Time in Patent Office: 2,329 Days
• Field of Search: 435/287.2, 435/287.9, 435/288.4, 435/288.7, 435/808, 436/172, 600/347, 422/82.05, 422/82.06, 422/82.07
• US Class Current: 435/288.7
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: Y10S 435/808 Optical sensing apparatus
• CPC Class Codes: Y10T 436/144444 Glucose

Abstract

A sensor probe suited for implanting into the skin of a person includes a sensor body which may be formed from a polymer which includes 2-hydroxyethyl methacrylate (HEMA). A sensing system is supported by the body. The sensing system exhibits a detectable change when the probe is exposed to the analyte in the fluid. The sensing system may include an enzyme capable of catalyzing a reaction of the analyte to form a reaction product and a dye system which absorbs in the infrared region of the spectrum in response to the reaction product.

Claims

1. A multivariable sensor system for simultaneously detecting one or more analytes in a fluid of a person, said sensor system comprising:

an elongated sensor body including an array of spaced apart cavities;

a plurality of optode sensing microbeads disposed in at least one cavity, said plurality of optode sensing microbeads comprising a first set of microbeads that include an indicator material and a detection substance;

a reference element disposed in at least one other cavity for providing a standard color by which a color change of said plurality of optode sensing microbeads is compared; and

a multilayered analyte-permeable membrane covering each of said cavities;

wherein said detection substance comprises an enzyme that reacts with the analyte or catalyzes the reaction of the analyte to produce a detectable reaction product;

wherein said at least one indicator material comprises a light-absorbing, pH-sensitive dye that undergoes a color change in response to the analyte or to the detectable reaction product thereof;

wherein the color change is reversible depending upon the concentration of the one or more analytes.

2. The sensor system of claim 1, wherein each of said cavities has an opaque or mirrored base to aid in viewing of said sensing microbeads without interference from underlying skin color.

3. The sensor system of claim 1, wherein said plurality of sensing microbeads include a second set of microbeads having at least one detection substance immobilized thereon.

4. The sensor system of claim 1 further including a third set of microbeads disposed in at least one of said spaced apart cavities, said third set of microbeads being colored to facilitate diffuse reflectance in said sensor system.

5. The sensor system of claim 1, wherein a negatively-charged hydrophilic gel is disposed in each of said spaced apart cavities, said negatively-charged hydrophilic gel including at least one polyanion to reduce the buffer capacity of said sensor system in vivo.

6. The sensor system of claim 1, wherein said multilayered analyte-permeable membrane is transparent.

7. The sensor system of claim 1, wherein said multilayered analyte-permeable membrane is permeable to select molecules.

8. The sensor system of claim 7, wherein said multilayered analyte-permeable membrane excludes at least one of anions, lipids and proteins.

9. The sensor system of claim 1, wherein said multilayered analyte permeable membrane further comprises:

an outermost layer that is in contact with the fluid;

a middle layer for regulating and limiting the diffusion of molecules into each of said spaced apart cavities; and

a negatively-charged inner layer that is in contact with said microbeads, said negatively-charged inner layer being formed from a combination of cellulose acetate and cellulose acetate phthalate in a ratio that allows the diffusion rate of charged ions into said at least one cavity including said plurality of sensing optode microbeads to be controlled.

10. The sensor system of claim 1, wherein each of said plurality of optode sensing microbeads is comprised of a lipophilic hydrogen ion-sensitive dye, an ionophore, and a lipophilic ion.

11. A multivariable sensor system for simultaneously detecting one or more analytes in a fluid of a person, said sensor system comprising:

an elongated sensor body including an array of spaced apart cavities;

a plurality of sensing optode microbeads disposed in at least one cavity, said plurality of sensing optode microbeads comprising a first set of microbeads that include an indicator material and a second set of microbeads that include a detection substance;

a plurality of reference microbeads disposed in at least one other cavity for providing a standard color by which a color change of said plurality of optode sensing microbeads is compared; and

a multilayered analyte-permeable membrane covering each of said cavities;

wherein said detection substance comprises an enzyme that reacts with the analyte or catalyzes the reaction of the analyte to produce a detectable reaction product;

wherein said at least one indicator material comprises a light-absorbing, pH-sensitive dye that undergoes a color change in response to the analyte or to the detectable reaction product thereof;

wherein the color change is reversible depending upon the concentration of the one or more analytes.