Wave guide with isolated coupling interface

• US 7,968,060 B2
• Filed: 08/29/2007
• Issued: 06/28/2011
• Est. Priority Date: 11/27/2002
• Status: Active Grant

• Current Assignee: Labcyte, Inc. (Danaher Corporation)
• Original Assignee: EDC Biosystems, Inc.
• Inventors: Van Tuyl, Michael
• Primary Examiner(s): Wright; P. Kathryn
• Assistant Examiner(s): TURK, NEIL N
• Application Number:
  US11/897,352
• Publication Number:
  US 20070296760A1
• Time in Patent Office: 1,399 Days
• Field of Search: None
• US Class Current: 422/501
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2400/027 electrostatic forces between substrate and tip
• CPC Class Codes: B01L 2400/0415 electrical forces, e.g. electrokinetic
• CPC Class Codes: B01L 2400/0436 acoustic forces, e.g. surface acoustic waves [SAW]
• CPC Class Codes: B01L 3/0268 using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries
• CPC Class Codes: B01L 9/523 for multisample carriers, e.g. used for microtitration plates
• CPC Class Codes: G01N 2035/1041 Ink-jet like dispensers
• CPC Class Codes: G01N 35/1072 with provision for selective pipetting of individual channels
• CPC Class Codes: G01N 35/1074 arranged in a two-dimensional array
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A wave-guide having an isolated coupling interface. In one variation, a constant negative pressure is maintained around the area surrounding the wave-guide. Coupling liquid may be directed to the tip of the wave-guide to provide the coupling interface between the wave-guide and a source fluid container. The suction from the constant negative pressure may remove excess coupling liquid and isolating the coupling liquid to the area around the tip of the wave-guide. The wave-guide assembly may also include mechanisms for adjusting the volume of fluid at the tip of the wave-guide. When the position of the wave-guide is displaced, fluid compensation mechanism may increase or decrease the volume of fluids at the distal end of the wave-guide to maintain proper coupling between the wave-guide and the source fluid container. Methods for utilizing negative pressure around the distal end of the wave-guide to isolate the coupling liquid are also described.

Claims

1. A wave-guide assembly comprising:

a wave-guide;

a structure having a suction channel for removing excess coupling liquid from the area surrounding a distal end of said wave-guide, wherein said wave-guide is moveably disposed within said structure; and

a fluid channel within said structure for supplying a coupling liquid to the distal end of said wave-guide;

wherein said structure comprises a cylindrical lumen and a hollow elongated cylinder positioned within said cylindrical lumen, wherein an inner surface of said cylindrical lumen and an outer surface of said hollow elongated cylinder define said suction channel; and

said wave-guide is moveably disposed within said hollow elongated cylinder, wherein an inner surface of said hollow elongated cylinder and an outer surface of said wave-guide define said fluid channel.

2. A wave-guide assembly of claim 1 further comprising:

a piezoelectric transducer attached to a proximal end of said wave-guide.

3. A wave-guide assembly of claim 2 wherein the distal end of said wave-guide has a lens-shaped tip.

4. A wave-guide assembly of claim 1 wherein said wave-guide is configured to eject a droplet of liquid about 10 micro-liter or less.

5. The wave-guide assembly of claim 1 further comprising:

a fluid source connected to said fluid channel.

6. The wave-guide assembly of claim 1 further comprising:

a suction generator connected to said suction channel.

7. The wave-guide assembly of claim 1 further comprising:

a suction source connected to said suction channel.

8. The wave-guide assembly of claim 6 further comprising:

a fluid pump connected to said fluid channel for supplying coupling liquid to the distal end of said wave-guide.

9. The wave-guide assembly of claim 8 further comprising:

a fluid displacement device connected to said fluid channel for adjusting a volume of coupling liquid at the distal end of said wave-guide.

10. The wave-guide assembly of claim 9 wherein said fluid displacement device is coupled to said wave-guide.

11. The wave-guide assembly of claim 9 wherein said fluid displacement device comprises a piston pump.

12. The wave-guide assembly of claim 11 further comprising:

a wave-guide support connected to said wave-guide, wherein a portion of said wave-guide support and said piston pump are connected to one another.

13. The wave-guide assembly of claim 8 further comprising:

a fluid reservoir connected to said fluid pump; and

a tubing connecting said suction generator to said fluid reservoir.

14. The wave-guide assembly of claim 6 further comprising:

said coupling liquid located within said fluid channel.

15. The wave-guide assembly of claim 14 wherein said coupling liquid consists essentially of water.

16. The wave-guide assembly of claim 1 wherein said structure further comprises a trough on an outer perimeter of said structure adapted for collecting fluids on an upper surface of said trough.

17. The wave-guide assembly of claim 16 further comprising:

a drain channel connecting said suction generator to the upper surface of said trough.

18. The wave-guide assembly of claim 16 further comprising:

a second suction generator connected to said upper surface of said trough.

19. The wave-guide assembly of claim 2 further comprising:

a frame coupled to said structure, wherein said frame is adapted to allow sufficient movement of the structure as said wave-guide is moved vertically to prevent binding of said wave-guide against said structure.

20. The wave-guide assembly of claim 19 further comprising:

a carriage connected to said frame for moving said frame and said structure in the vertical direction.

21. The wave-guide assembly of claim 2 further comprising:

a support coupled to said structure, wherein said supporting apparatus is adapted to allow sufficient movement of the structure as said wave-guide is moved vertically to prevent binding of said wave-guide against said structure.

22. The wave-guide assembly of claim 21 further comprising:

a linear displacement device coupled to said supporting apparatus for moving said supporting apparatus in the vertical direction.

23. The wave-guide assembly of claim 1 further comprising:

a frame, wherein said structure is coupled to said frame such that said structure may move along the X/Y plane of said frame and not along the Z-axis of said frame.

24. The wave-guide assembly of claim 23, wherein the movement along the X/Y plane is limited to about 1 mm along the X-axis and about 1 mm along the Y-axis.

25. The wave-guide assembly of claim 1 further comprising:

a stage for holding and moving a fluid container above said structure, said stage is adapted to maintain a constant vertical distance between said structure and said fluid container while said stage is moving in an X/Y plane.

26. The wave-guide assembly of claim 25 further comprising:

a fluid container having a plurality of wells, wherein said fluid container is placed on said stage.

27. The wave-guide assembly of claim 26 further comprising:

a coupling liquid, wherein said coupling liquid is in contact with said wave-guide and the bottom surface of said fluid container.

28. The wave-guide assembly of claim 1 wherein said wave-guide assembly is configured to maintain a liquid contact with a fluid containment structure as the wave-guide moves vertically.

29. The wave-guide assembly of claim 1 further comprising:

a trough surrounding said first cylindrical body, wherein said trough is connected to said cylindrical body;

a fluid pump connected to said fluid channel;

a negative pressure generator connected to said suction channel;

a piston pump connected to said fluid channel;

a piezoelectric transducer connected to said wave-guide;

a frame, wherein said structure is coupled to said frame such that said structure may move in an X-Y plane of said frame but may not move in the Z direction; and

a motor coupled to said wave-guide for moving the wave-guide in a vertical direction within said structure.

30. The wave-guide assembly of claim 1 wherein said fluid channel surrounds said wave-guide, and said suction channel surrounds said fluid channel.

31. A wave-guide assembly of claim 30 further comprising:

a fluid source connected to said fluid channel; and

a negative pressure generator connected to said suction channel.

32. The wave-guide assembly of claim 31 further comprising:

an acoustic wave emitter connected to said wave-guide.

33. The wave-guide assembly of claim 31 further comprising:

a trough surrounding said suction channel.

34. The wave-guide assembly of claim 33 further comprising:

a drainage channel within said structure, said drainage channel is connected to said trough for draining fluids from said trough.

35. The wave-guide assembly of claim 32 further comprising:

a coupling liquid, wherein said coupling liquid is positioned on the distal end of said wave-guide.

36. The wave-guide assembly of claim 35 further comprising:

a fluid container having a bottom surface and moveably disposed above said wave-guide such that said coupling liquid is positioned between said wave-guide and said fluid container, and said coupling liquid comes into contact with both the wave-guide and the bottom surface of said fluid container, said fluid container further having a plurality of wells located in said fluid container.

37. The wave-guide assembly of claim 32 further comprising:

a fluid displacement device connected to said fluid channel for displacing a volume of said coupling liquid at a distal tip of said wave-guide.

38. The wave-guide assembly of claim 37 wherein said fluid source comprises a fluid pump connected to a fluid reservoir.

39. The wave-guide assembly of claim 1 wherein said fluid channel has an outlet located next to the distal end of said wave-guide, and said suction channel has an inlet located next to the outlet of said fluid channel.

40. The wave-guide assembly of claim 39 further comprising:

a fluid source connected to said fluid channel; and

a suction generator connected to said suction channel.

41. The wave-guide assembly of claim 40 wherein the inlet of said suction channel surrounds the distal end of said wave-guide.

42. The wave-guide assembly of claim 1 wherein said structure has a lumen, a distal end and a proximal end, a cavity located next to an opening of said lumen at the distal end of said structure, said fluid channel running from said lumen to an inlet, said suction channel running from said cavity to an outlet, said wave-guide is moveably disposed within said lumen.

43. The wave-guide assembly of claim 42 further comprising:

a negative pressure generator connected to said outlet;

a fluid source connected to said inlet; and

an acoustic wave generator connected to said wave-guide.

44. The wave-guide assembly of claim 43 further comprising:

a frame, wherein said structure is coupled to said frame such that said structure may only move in the X axis and Y axis direction and not in the Z axis direction.

45. The wave-guide assembly of claim 1 further comprising:

a fluid displacement device connected to said fluid channel, wherein said fluid displacement device is adapted to adjust the volume of coupling liquid at the distal end of said wave-guide.

46. The wave-guide assembly of claim 1 further comprising:

a piston pump coupled to said wave-guide.