Methods of isolation, expansion and differentiation of fetal stem cells from chorionic villus, amniotic fluid, and placenta and therapeutic uses thereof

• US 7,968,336 B2
• Filed: 11/28/2006
• Issued: 06/28/2011
• Est. Priority Date: 11/15/2001
• Status: Expired due to Fees

• Current Assignee: Children's Medical Center Corporation
• Original Assignee: Children's Medical Center Corporation
• Inventors: De Coppi, Paolo, Atala, Anthony
• Primary Examiner(s): WEHBE, ANNE MARIE SABRINA
• Application Number:
  US11/604,917
• Publication Number:
  US 20070116683A1
• Time in Patent Office: 1,673 Days
• Field of Search: 435/366, 435/325
• US Class Current: 435/366
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61P 1/16 for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
• CPC Class Codes: A61P 19/08 for bone diseases, e.g. rachitism, Paget's disease
• CPC Class Codes: A61P 21/00 Drugs for disorders of the muscular or neuromuscular system
• CPC Class Codes: A61P 25/00 Drugs for disorders of the nervous system
• CPC Class Codes: A61P 25/16 Anti-Parkinson drugs
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: A61P 7/00 Drugs for disorders of the blood or the extracellular fluid
• CPC Class Codes: C12N 5/0605 Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
• CPC Class Codes: C12N 5/0607 Non-embryonic pluripotent stem cells, e.g. MASC induced pluripotent stem cells C12N5/0696

Abstract

The present invention is directed to pluripotent fetal stem cells derived from chorionic villus, amniotic fluid, and placenta and the methods for isolating, expanding and differentiating these cells, and their therapeutic uses such as manipulating the fetal stem cells by gene transfection and other means for therapeutic applications.

Claims

1. A composition enriched for isolated human fetal stem cells that are c-kit positive and at least one of SSEA3 positive and SSEA4 positive, wherein the isolated human fetal stem cells grow without a feeder layer and do not spontaneously differentiate in culture, and are isolated from a sample of chorionic villus of the human placenta.

2. The composition according to claim 1, wherein said stem cells are c-kit positive, SSEA3 positive, and SSEA4 positive.

3. The composition according to claim 1, wherein said stem cells are SSEA1 negative.

4. The composition according to claim 2, wherein said stem cells are SSEA1 negative.

5. An isolated pluripotent human fetal stem cell, which is c-kit positive, and at least one of SSEA3 positive and SSEA4 positive, that grows without a feeder layer and does not spontaneously differentiate in culture, from a sample of chorionic villus of the human placenta.

7. The isolated pluripotent human fetal stem cell according to claim 5, which is SSEA1 negative.

6. An isolated pluripotent human fetal stem cell, which is c-kit positive, SSEA3 positive, and SSEA4 positive, that grows without a feeder layer and does not spontaneously differentiate in culture, from a sample of chorionic villus of the human placenta.

8. The isolated pluripotent human fetal stem cell according to claim 6, which is SSEA1 negative.