Neural precursor cells, method for the production and use thereof in neural defect therapy
First Claim
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1. A method for the generation of an isolated, non-tumorigenic cell composition consisting essentially of embryonic stem cell-derived neural precursor cells, and neuronal cells derived from the embryonic stem cell-derived neural precursor cells comprising:
- (a) culturing mouse or human embryonic stem cells that are not human genetically modified embryonic stem cells to produce neural precursor cells;
(b) culturing the neural precursor cells from (a) in a first growth factor-containing serum-free medium, the first medium comprising basic fibroblast growth factor (bFGF);
(c) culturing the cells from (b) in a second growth factor-containing serum-free medium, the second medium comprising bFGF and epidermal growth factor (EGF); and
(d) culturing the cells from (c) in a third growth factor-containing serum-free medium, the third medium comprising bFGF and platelet-derived growth factor (PDGF), to obtain the cell composition consisting essentially of embryonic stem cell-derived neural precursor cells, and neuronal cells derived from the embryonic stem cell-derived neural precursor cells.
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Abstract
The invention relates to isolated and purified neural precursor cells, to methods for the generation of such precursor cells in unlimited quantities from embryonic stem cells, and to their use for the therapy of neural defects, particularly in mammals, preferably in human beings, and for the generation of polypeptides.
7 Citations
16 Claims
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1. A method for the generation of an isolated, non-tumorigenic cell composition consisting essentially of embryonic stem cell-derived neural precursor cells, and neuronal cells derived from the embryonic stem cell-derived neural precursor cells comprising:
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(a) culturing mouse or human embryonic stem cells that are not human genetically modified embryonic stem cells to produce neural precursor cells; (b) culturing the neural precursor cells from (a) in a first growth factor-containing serum-free medium, the first medium comprising basic fibroblast growth factor (bFGF); (c) culturing the cells from (b) in a second growth factor-containing serum-free medium, the second medium comprising bFGF and epidermal growth factor (EGF); and (d) culturing the cells from (c) in a third growth factor-containing serum-free medium, the third medium comprising bFGF and platelet-derived growth factor (PDGF), to obtain the cell composition consisting essentially of embryonic stem cell-derived neural precursor cells, and neuronal cells derived from the embryonic stem cell-derived neural precursor cells. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A method for the generation of an isolated, non-tumorigenic cell composition comprising neural spheres, wherein the neural spheres consist essentially of embryonic stem cell-derived neural precursor cells, and neuronal cells derived from the embryonic stem cell-derived neural precursor cells comprising:
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(a) culturing mouse or human embryonic stem cells that are not human genetically modified embryonic stem cells to produce neural precursor cells; (b) culturing the neural precursor cells from (a) in a first growth factor containing serum-free medium, the first medium comprising bFGF; (c) culturing the cells from (b) in a second growth factor-containing serum-free medium, the second medium comprising bFGF and EGF to produce neural spheres consisting essentially of embryonic stem cell-derived neural precursor cells, and neuronal cells derived from the embryonic stem cellderived neural precursor cells, thereby producing the isolated, non-tumorigenic cell composition comprising neural spheres. - View Dependent Claims (9, 10, 11, 12, 13, 14, 15, 16)
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