Methods and compositions relating to the regulation of MUC1 by HSF1 and STAT3

• US 7,972,870 B2
• Filed: 02/01/2008
• Issued: 07/05/2011
• Est. Priority Date: 02/02/2007
• Status: Expired due to Fees

• Current Assignee: Dana-Farber Cancer Institute
• Original Assignee: Dana-Farber Cancer Institute
• Inventors: Kufe, Donald W.
• Primary Examiner(s): GUSSOW, ANNE
• Application Number:
  US12/024,692
• Publication Number:
  US 20090092600A1
• Time in Patent Office: 1,250 Days
• Field of Search: None
• US Class Current: 436/503
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: G01N 2333/4706 stimulating, promoting or activating activity
• CPC Class Codes: G01N 2333/4725 Mucins, e.g. human intestinal mucin
• CPC Class Codes: G01N 2500/02 Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)
• CPC Class Codes: G01N 33/5008 for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
• CPC Class Codes: G01N 33/6845 Methods of identifying protein-protein interactions in protein mixtures

Abstract

This invention relates to regulation of cell signaling, cell growth, and more particularly to the regulation of cancer or inflammatory cell growth and/or activation. The invention provides methods of inhibiting interactions between MUC1 and a heat shock factor, method of inhibiting interactions between transcription factors and the MUC1 promoter, and methods of inhibiting MUC1 expression. The invention also provides screening methods for identifying compounds that inhibit the aforementioned interactions. Pharmaceutical compositions containing the identified compounds can be useful in treating cancers and inflammatory conditions.

Claims

1. A method of identifying a compound that inhibits the binding of MUC1 to STAT3, the method comprising:

(a) contacting a MUC1 reagent comprising SEQ ID NO;

2 with a STAT3 reagent comprising SEQ ID NO;

5 in the presence of a candidate compound; and

(b) determining whether the candidate compound inhibits binding of the MUC1 reagent to the STAT3 reagent.