Compositions and methods for modulating pigment production in plants

• US 7,973,216 B2
• Filed: 08/30/2006
• Issued: 07/05/2011
• Est. Priority Date: 08/30/2005
• Status: Active Grant

• Current Assignee: The New Zealand Institute For Plant and Food Research Limited
• Original Assignee: The New Zealand Institute For Plant and Food Research Limited
• Inventors: Hellens, Roger, Allan, Andrew C., Espley, Richard
• Primary Examiner(s): Kumar; Vinod
• Application Number:
  US12/065,251
• Publication Number:
  US 20110072539A1
• Time in Patent Office: 1,770 Days
• Field of Search: None
• US Class Current: 800/295
• CPC Class Codes: C07K 14/415 from plants
• CPC Class Codes: C12N 15/8217 Gene switch
• CPC Class Codes: C12N 15/8237 Externally regulated expression systems
• CPC Class Codes: C12N 15/8238 chemically inducible, e.g. tetracycline
• CPC Class Codes: C12N 15/825 involving pigment biosynthesis

Abstract

This invention relates to polynucleotides encoding novel transcription factors and to the encoded transcription factors, that are capable of regulating anthocyanin production in plants. The invention also relates to constructs and vectors comprising the polynucleotides, and to host cells, plant cells and plants transformed with the polynucleotides, constructs and vectors. The invention also relates to methods of producing plants with altered anthocyanin production and plants by the methods.

Claims

1. An isolated polynucleotide comprising a nucleotide sequence encoding a polypeptide having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:

1, wherein % identity is calculated over the whole length of the amino acid sequence, and wherein the polypeptide is a transcription factor that increases anthocyanin production upon expression in a plant.

2. The isolated polynucleotide of claim 1, wherein the polypeptide has the amino acid sequence of SEQ ID NO:

1.

3. The isolated polynucleotide of claim 1, wherein the nucleotide sequence encoding the polypeptide has at least 90% sequence identity to the coding nucleotide sequence of SEQ ID NO:

5 or SEQ ID NO;

102.

4. The isolated polynucleotide of claim 1, wherein the nucleotide sequence encoding the polypeptide has at least 90% sequence identity to the coding nucleotide sequence of SEQ ID NO:

5.

5. The isolated polynucleotide of claim 1, wherein the nucleotide sequence encoding the polypeptide has the coding nucleotide sequence of SEQ ID NO:

5.

6. A genetic construct comprising the polynucleotide of claim 1.

7. A host cell transformed with the polynucleotide of claim 1 to express said polypeptide.

8. A plant cell or plant transformed with the polynucleotide of claim 1 to express said polypeptide, and wherein expression of said polypeptide increases anthocyanin production in said transformed plant cell or plant.

15. A method for producing a plant cell or plant with increased anthocyanin production, the method comprising the steps of transformation of a plant cell or plant with the polynucleotide of claim 1 and expression of the polypeptide encoded by said polynucleotide in the transformed plant cell or plant, and wherein expression of said polypeptide increases anthocyanin production in said transformed plant cell or plant.

16. A plant produced by the method of claim 15.

9. An isolated polynucleotide comprising a nucleotide sequence encoding a polypeptide having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:

1, wherein % identity is calculated over the whole length of the amino acid sequence, and wherein the polypeptide is a transcription factor that up-regulates promoter of a gene involved in the anthocyanin biosynthetic pathway.

10. The polynucleotide of claim 9 wherein the gene encodes dihydroflavolon 4-reductase (DFR).

11. The polynucleotide of claim 9 wherein the gene encodes chalcone synthase (CHS).

12. A genetic construct comprising the polynucleotide of claim 9.

13. A host cell transformed with the polynucleotide of claim 9 to express said polypeptide.

14. A plant cell or plant transformed with the polynucleotide of claim 9 to express said polypeptide, and wherein expression of said polypeptide increases anthocyanin production in said transformed plant cell or plant.