Method and apparatus for interfacial sensing

• US 7,975,547 B2
• Filed: 11/23/2005
• Issued: 07/12/2011
• Est. Priority Date: 11/23/2004
• Status: Active Grant

• Current Assignee: Drexel University
• Original Assignee: Drexel University
• Inventors: Lec, Ryszard M
• Primary Examiner(s): Saint Surin; Jacques M
• Application Number:
  US11/719,895
• Publication Number:
  US 20090165559A1
• Time in Patent Office: 2,057 Days
• Field of Search: 735/79, 735/99, 736/02, 735/97, 702/106, 702/191, 702/56, 702/183, 702/185, 381/71.1, 381/71.2, 381/73.1, 436/527, 436/518
• US Class Current: 73/579
• CPC Class Codes: G01N 33/53 Immunoassay; Biospecific binding assay; Materials therefor

Abstract

A sensor is disclosed for analyzing information, such as interfacial interactions, found in various systems. A sensor is located proximate to an interface that is to be studied. The sensor is actuated in order to produce acoustical signals. The frequency of oscillation of the sensor is varied in order to enable the sensor to produce acoustical signals at different harmonics. The different acoustical signals are used to analyze the system at various distances from the surface of the sensor.

Claims

1. A sensor system comprising:

a sensor; and

an actuator for actuating said sensor;

said actuator being enabled for operation at a first operating condition of said sensor when placed proximate to a sample, and wherein said actuator is enabled for a second operating condition of the sensor when placed proximate to the sample.

2. The sensor system of claim 1, wherein said actuator is enabled for operation at a plurality of frequencies at which said sensor is actuated.

3. The sensor system of claim 1, wherein said actuator is enabled for operation at a plurality of amplitude at which said sensor is actuated.

4. The sensor system of claim 1, wherein said actuator is enabled for operation at a plurality of phases at which said sensor is actuated.

5. The sensor system of claim 1, wherein the sensor is an acoustic sensor comprising a piezoelectric material.

6. The sensor system of claim 1, further comprising a signal processor that compares a response parameter of the sensor to another instance of that response parameter.

7. The sensor system of claim 1, further comprising a signal processor that compares a response parameter of the sensor to a reference signature response.

8. The sensor system of claim 1, further comprising a functionalized interfacial surface.

9. The sensor system of claim 8, wherein the interfacial surface is functionalized with antibodies attached to the sensor surface.

10. The sensor system of claim 8, wherein the interfacial surface is functionalized with cells attached to the sensor surface.

11. The sensor system of claim 1, wherein said actuator is enabled for exciting the sensor at two or more frequencies, simultaneously.

12. The sensor system of claim 1, wherein said actuator is enabled for excitation at a fundamental resonant frequency and at least one higher harmonic of said fundamental resonant frequency.

13. The sensor system of claim 1 having a plurality of sensors.

14. The sensor system of claim 13, wherein a plurality of actuators are enabled for exciting said plurality of sensors.

15. A sensor system, comprising:

a sensor;

an actuator for actuating said sensor to cause an acoustic response at said sensor, said actuator configured for variation of an operating condition of the sensor when the sensor is placed proximate to a sample; and

a signal processor for capturing the acoustic response.

16. The sensor system of claim 15, wherein the signal processor captures one or more response parameters of said acoustic response.

17. The sensor system of claim 16, wherein the signal processor captures the response frequency of said acoustic response.

18. The sensor system of claim 16, wherein the signal processor captures the response amplitude of said acoustic response.

19. The sensor system of claim 16, wherein the signal processor captures the response phase of said acoustic response.

20. The sensor system of claim 16, wherein the signal processor captures the response Q-factor of said acoustic response.

21. The sensor system of claim 16, wherein the signal processor captures the response impedance of said acoustic response.

22. The sensor system of claim 15, wherein the signal processor captures two or more response parameters of said acoustic response.

23. The sensor system of claim 15, wherein the signal processor compares a response parameter of the sensor to another instance of that response parameter.

24. The sensor system of claim 15, wherein the signal processor compares a response parameter of the sensor to a reference signature response to characterize a biological process.

25. The sensor system of claim 15, wherein said sensor is configured to have a fundamental frequency selected to match the resonant frequency of a target particle.

26. The sensor system of claim 15, wherein the actuator is configured to actuate the sensor at an amplitude selected to nondestructively interrogate the sensor interface.

27. The sensor system of claim 15, wherein the actuator is configured to actuate the sensor at an amplitude selected to destructively interrogate the sensor interface.

28. The sensor system of claim 15, further comprising a preprocessor for selectively introducing a sample to said sensor.

29. The sensor system of claim 28, wherein the preprocessor comprises a selective membrane.

30. The sensor system of claim 28, wherein the preprocessor comprises an acoustic field.

31. The sensor system of claim 28, wherein the preprocessor comprises a magnetic field.

32. The sensor system of claim 28, wherein the preprocessor comprises an electric field.

33. The sensor system of claim 28, wherein the preprocessor comprises an optical field.

34. The sensor system of claim 28, wherein the preprocessor comprises a thermal gradient.

35. A method for sensing comprising the steps of:

providing a sensor proximate a sample to be sensed;

exciting the sensor at a first operating condition;

measuring a resonance feature of said sensor;

changing the operating condition of said sensor to at least one additional operating condition, and

measuring the resonance feature of said sensor at said at least one additional operating condition.

36. The method of claim 35, wherein the resonance feature is resonant frequency.

37. The method of claim 35, wherein the resonance feature is amplitude.

38. The method of claim 35, wherein the resonance feature is phase.

39. The method of claim 35, wherein the resonance feature is Q-factor.

40. The method of claim 35, wherein the first operating condition is the fundamental frequency of the sensor and sample system, and the at least one additional operating condition is a higher order harmonic of the fundamental frequency of the sensor and sample system.

41. The method of claim 35, further comprising the steps of measuring a second resonance feature at the first operating condition and measuring the second resonance feature at the at least one additional operating condition.

42. The method of claim 35, further comprising the step of:

comparing the resonance feature measurements to resonance feature measurements taken at a different time at the same operating condition.

43. The method of claim 35, further comprising the step of:

comparing the resonance feature measurements to a reference signature.

44. A method for sensing comprising the steps of:

selecting an interrogation distance from a surface of an excitable sensor,

exciting the sensor at a frequency that interrogates at the selected interrogation

distance from said surface of said sensor, and

measuring a resonance feature of said sensor.

45. The method of claim 44, wherein the resonance feature is resonant frequency.

46. The method of claim 44, wherein the resonance feature is amplitude.

47. The method of claim 44, wherein the resonance feature is phase.

48. The method of claim 44, wherein the resonance feature is Q-factor.

49. The method of claim 44, further comprising the step of measuring a second resonance feature of said sensor.

50. A method for sensing comprising the steps of;

providing a sensor with known mass and fundamental frequency proximate a sample containing a particle to be sensed;

exciting the sensor at a fundamental frequency of the sensor coupled to the particle;

measuring the resonant frequency of said coupled sensor and particle;

calculating the diameter of the particle coupled to the sensor using the measured resonant frequency.