Generation and isolation of antigen-specific T cells
First Claim
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1. A method for generating and/or enriching antigen-specific T cells comprising:
- (a) exposing a first population of cells wherein at least a portion thereof comprises antigen presenting cells to a paramagnetic bead wherein said paramagnetic bead has antigen attached thereto, such that said paramagnetic bead with antigen attached thereto is ingested by said APC;
(b) exposing a second population of cells wherein at least a portion thereof comprises T cells to the population of cells in part (a);
thereby generating and/or enriching antigen-specific T cells through direct contact between said APC and said T cells; and
(c) isolating said APCs and antigen-specific T cells by means of a magnetic field.
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Abstract
The present invention relates generally to methods for generating, expanding, and isolating antigen-specific T cells. Compositions of antigen-specific T cells activated and expanded by the methods herein are further provided.
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14 Claims
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1. A method for generating and/or enriching antigen-specific T cells comprising:
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(a) exposing a first population of cells wherein at least a portion thereof comprises antigen presenting cells to a paramagnetic bead wherein said paramagnetic bead has antigen attached thereto, such that said paramagnetic bead with antigen attached thereto is ingested by said APC; (b) exposing a second population of cells wherein at least a portion thereof comprises T cells to the population of cells in part (a);
thereby generating and/or enriching antigen-specific T cells through direct contact between said APC and said T cells; and(c) isolating said APCs and antigen-specific T cells by means of a magnetic field. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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