Analysis method and analysis apparatus

• US 7,977,116 B2
• Filed: 03/12/2007
• Issued: 07/12/2011
• Est. Priority Date: 03/17/2006
• Status: Active Grant

• Current Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Original Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Inventors: Kasai, Shintaro, Yoneyama, Haruko, Yamashita, Masatsugu, Ouchi, Toshihiko
• Primary Examiner(s): Menon; Krishnan S
• Assistant Examiner(s): Gionta; Allison
• Application Number:
  US11/716,702
• Publication Number:
  US 20070229094A1
• Time in Patent Office: 1,583 Days
• Field of Search: 436/6, 436/64, 436/161, 436/162
• US Class Current: 436/164
• CPC Class Codes: G01N 21/3577 for analysing liquids, e.g. polluted water
• CPC Class Codes: G01N 21/3581 using far infrared light; using Terahertz radiation

Abstract

Provided are an analysis method and an analysis apparatus that can perform analysis of a substance and information obtainment with relatively high accuracy and reproducibility without previously allowing a carrier to carry a reagent for a color reaction. In the analysis method and the analysis apparatus, the information on an analyte is obtained by using an electromagnetic wave of a frequency including a frequency band which is at least a part of a frequency range of 30 GHz or more and 30 THz or less. A non-fibrous, isotropic porous material is allowed to hold the analyte, the analyte held by the porous material is irradiated with the electromagnetic wave, a change in the propagation state of the electromagnetic wave due to transmission through or reflection by the porous material is detected and information on the analyte is obtained based on the result of the detection.

Claims

1. An analysis method of obtaining information on an analyte by using an electromagnetic wave of a frequency including a frequency band which is at least a part of a frequency range of 30 GHz or more and 30 THz or less, the method comprising:

holding a liquid comprising an analyte in a membrane filter;

irradiating the analyte held in the membrane filter with an electromagnetic wave; and

detecting a change in propagation state of the electromagnetic wave due to transmission through or reflection by the membrane filter, and obtaining information about the analyte based on a result of the detection,

wherein the membrane filter comprises a non-fibrous, isotropic porous material,

wherein an average pore diameter of a plurality of pores of the porous material is 0.1 μ

m or more, and

wherein the analyte is held while being entangled with the plurality of pores of the porous material.

2. The analysis method according to claim 1, wherein the porous material comprises at least one selected from the group consisting of polypropylene, polysulfone, nylon, polyethersulfone, Teflon, polyolefin, polyethylene, polystyrene, and ethylene tetrafluoride resins.

3. The analysis method according to claim 1, wherein after a solution or lysate of a biomolecule is held in the membrane filter and then subjected to drying, the information is obtained.

4. The analysis method according to claim 1, wherein after a biomolecule is held in the membrane filter and the information is obtained, the biomolecule is eluted from the membrane filter for reuse.

5. The analysis method according to claim 1, wherein when detecting the change in the propagation state of the electromagnetic wave due to the reflection by the membrane filter, a member that totally reflects the electromagnetic wave is used, the membrane filter is disposed in the vicinity of a reflective surface of the totally reflecting member, and the information on the analyte held in the membrane filter is obtained.

6. The analysis method according to claim 1, wherein a plurality of the membrane filters at least one of which holds the analyte are disposed on a rotary member rotatable about a center in a plane not parallel to the travel direction of the electromagnetic wave, at locations radially apart by the same distance from the center, the membrane filters are sequentially brought into a detection position by rotating the rotary member, and the change in the propagation state of the electromagnetic wave due to transmission through or reflection by the membrane filters brought into the detection position is sequentially detected.

7. The analysis method according to claim 1, wherein the porous material has an electromagnetic wave amplitude transmittance of 90% or more at 2.0 THz.

8. The analysis method according to claim 1, wherein when an index indicating constancy of the amplitude transmittance with respect to frequency in the frequency band of the electromagnetic wave is defined as the absolute value of a value obtained by subtracting 1 from the amplitude transmittance TM of a frequency near the middle of a terahertz wave frequency band used in the measurement and dividing the resulting value by a difference between the amplitude transmittance TH at the upper limit of the frequency band and the amplitude transmittance TL at the lower limit of the frequency band, the porous material has the index of 20 or more.

9. An analysis apparatus for performing the analysis method set forth in claim 1, comprising an electromagnetic wave irradiating unit for irradiating the membrane filter with the electromagnetic wave, and an electromagnetic wave detecting unit for detecting the change in the propagation state of the electromagnetic wave due to the transmission through or the reflection by the membrane filter.

10. The analysis method according to claim 1, wherein the average pore diameter is 2.0 μ

m or less.

11. The analysis method according to claim 1, wherein the porous material comprises at least one selected from the group consisting of polypropylene, polysulfone, nylon, and polyethersulfone.