Chemical arrays and methods of using the same

• US 7,977,119 B2
• Filed: 12/08/2004
• Issued: 07/12/2011
• Est. Priority Date: 12/08/2004
• Status: Active Grant

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Agilent Technologies, Inc.
• Inventors: Leproust, Eric M., Kronick, Mel N.
• Primary Examiner(s): Jung; Unsu
• Application Number:
  US11/008,384
• Publication Number:
  US 20060121543A1
• Time in Patent Office: 2,407 Days
• Field of Search: 435 76- 772, 435/7.9, 436/532, 530/334, 530/335, 530/344
• US Class Current: 436/518
• CPC Class Codes: C40B 30/04 by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing

Abstract

Methods and compositions for generating mixtures of product molecules from an initial chemical array are provided. In the subject methods, a chemical array of surface immobilized first moieties is subjected to cleavage conditions such that a composition of solution phase first moieties is produced. The resultant composition of solution phase first moieties is then contacted with one or more reactants to produce a mixture of product molecules that are different from the first moieties. Also provided are the arrays employed in the subject methods and kits for practicing the subject methods.

Claims

1. A method comprising:

(a) subjecting an array of a plurality of polypeptide or polysaccharide features comprising polypeptide or polysaccharide first moieties immobilized on a surface of a solid support via a cleavable domain to conditions sufficient to cleave said cleavable domain and generate a solution phase composition of said polypeptide or polysaccharide first moieties, wherein said solution phase composition is a mixture comprising two or more of said polypeptide or polysaccharide first moieties; and

(b) contacting said solution phase composition of said polypeptide or polysaccharide first moieties with one or more reactants to produce a mixture of product molecules that are different from said polypeptide or polysaccharide first moieties.

2. The method according to claim 1, wherein said array is an array of a plurality of polysaccharide features.

3. The method according to claim 1, wherein said cleavable domain is base labile domain.

4. The method according to claim 1, wherein said cleavable linker is an acid labile linker.

5. The method according to claim 1, wherein said one or more reactants comprises an enzymatic activity.

6. The method according to claim 5, wherein said product molecules are polypeptides.

7. A method comprising:

(a) subjecting an array of a plurality of polypeptide features each comprising first polypeptides immobilized on a surface of a solid support via a cleavable domain to conditions sufficient to cleave said cleavable domain and generate a solution phase composition of said first polypeptides, wherein said solution phase composition is a mixture comprising two or more of said first polypeptides; and

(b) contacting said solution phase composition of said first polypeptides with an enzymatic reagent system to produce a mixture of product polypeptides that are different from said first polypeptides.

8. The method according to claim 7, wherein said cleavable domain comprises base labile region.

9. The method according to claim 7, wherein said cleavable domain comprises an acid labile region.