Chemical arrays and methods of using the same
First Claim
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1. A method comprising:
- (a) subjecting an array of a plurality of polypeptide or polysaccharide features comprising polypeptide or polysaccharide first moieties immobilized on a surface of a solid support via a cleavable domain to conditions sufficient to cleave said cleavable domain and generate a solution phase composition of said polypeptide or polysaccharide first moieties, wherein said solution phase composition is a mixture comprising two or more of said polypeptide or polysaccharide first moieties; and
(b) contacting said solution phase composition of said polypeptide or polysaccharide first moieties with one or more reactants to produce a mixture of product molecules that are different from said polypeptide or polysaccharide first moieties.
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Abstract
Methods and compositions for generating mixtures of product molecules from an initial chemical array are provided. In the subject methods, a chemical array of surface immobilized first moieties is subjected to cleavage conditions such that a composition of solution phase first moieties is produced. The resultant composition of solution phase first moieties is then contacted with one or more reactants to produce a mixture of product molecules that are different from the first moieties. Also provided are the arrays employed in the subject methods and kits for practicing the subject methods.
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Citations
9 Claims
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1. A method comprising:
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(a) subjecting an array of a plurality of polypeptide or polysaccharide features comprising polypeptide or polysaccharide first moieties immobilized on a surface of a solid support via a cleavable domain to conditions sufficient to cleave said cleavable domain and generate a solution phase composition of said polypeptide or polysaccharide first moieties, wherein said solution phase composition is a mixture comprising two or more of said polypeptide or polysaccharide first moieties; and (b) contacting said solution phase composition of said polypeptide or polysaccharide first moieties with one or more reactants to produce a mixture of product molecules that are different from said polypeptide or polysaccharide first moieties. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A method comprising:
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(a) subjecting an array of a plurality of polypeptide features each comprising first polypeptides immobilized on a surface of a solid support via a cleavable domain to conditions sufficient to cleave said cleavable domain and generate a solution phase composition of said first polypeptides, wherein said solution phase composition is a mixture comprising two or more of said first polypeptides; and (b) contacting said solution phase composition of said first polypeptides with an enzymatic reagent system to produce a mixture of product polypeptides that are different from said first polypeptides. - View Dependent Claims (8, 9)
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Specification