Optimized method for LID biosensor resonance detection
First Claim
1. An optical interrogation system comprising:
- a launch system that emits an optical beam towards a biosensor;
a receive system that collects an optical beam from the biosensor and then outputs a signal which corresponds to the collected optical beam;
a processor that applies a low pass filter to the signal to digitally remove a plurality of problematic parasitic reflections located within the signal that represents an optical resonance; and
an optical isolator located between the biosensor and both a lensed fiber of the launch system and a lensed fiber of the receive system, wherein the optical isolator filters out the parasitic reflections caused by a part of the optical beam reflected from a face of a substrate of the biosensor, and wherein the optical isolator does not filter out the parasitic reflections caused by the optical beam which passes through the substrate and is reflected from a top surface of the biosensor.
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Accused Products
Abstract
An optical interrogation system is described herein that can interrogate a label-independent-detection (LID) biosensor and monitor a biological event on top of the biosensor without suffering from problematical parasitic reflections and/or problematical pixelation effects. In one embodiment, the optical interrogation system is capable of interrogating a biosensor and using a low pass filter algorithm to digitally remove problematic parasitic reflections contained in the spectrum of an optical resonance which makes it easier to determine whether or not a biological event occurred on the biosensor. In another embodiment, the optical interrogation system is capable of interrogating a biosensor and using an oversampling/smoothing algorithm to reduce oscillations in the estimated location of an optical resonance caused by the problematical pixelation effect which makes it easier to determine whether or not a biological event occurred on the biosensor.
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Citations
22 Claims
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1. An optical interrogation system comprising:
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a launch system that emits an optical beam towards a biosensor; a receive system that collects an optical beam from the biosensor and then outputs a signal which corresponds to the collected optical beam; a processor that applies a low pass filter to the signal to digitally remove a plurality of problematic parasitic reflections located within the signal that represents an optical resonance; and an optical isolator located between the biosensor and both a lensed fiber of the launch system and a lensed fiber of the receive system, wherein the optical isolator filters out the parasitic reflections caused by a part of the optical beam reflected from a face of a substrate of the biosensor, and wherein the optical isolator does not filter out the parasitic reflections caused by the optical beam which passes through the substrate and is reflected from a top surface of the biosensor. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. An optical interrogation system comprising:
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a launch system that emits an optical beam towards a biosensor; a receive system that collects an optical beam from the biosensor and then outputs a signal which corresponds to the collected optical beam; and a processor that applies a low pass filter to the signal to digitally remove a plurality of problematic parasitic reflections located within the signal that represents an optical resonance, wherein the low pass filter filters out the parasitic reflections caused by the optical beam which passes through a substrate of the biosensor and is then reflected from a top surface of the biosensor, and wherein the low pass filter does not filter out the parasitic reflections caused by a part of the optical beam reflected from a face of the substrate of the biosensor. - View Dependent Claims (12)
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13. A method for interrogating a biosensor, said method comprising the steps of:
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emitting an optical beam towards the biosensor; collecting an optical beam from the biosensor; generating a signal which corresponds to the collected optical beam; applying a low pass filter to the signal to digitally remove a plurality of problematic parasitic reflections located within the signal that represents an optical resonance; and using an optical isolator to filter out the parasitic reflections caused by a part of the optical beam reflected from a face of a substrate of the biosensor while the optical isolator does not filter out the parasitic reflections caused by the optical beam which passes through the substrate and is reflected from a top surface of the biosensor. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20)
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21. A method for interrogating a biosensor, said method comprising the steps of:
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emitting an optical beam towards the biosensor; collecting an optical beam from the biosensor; generating a signal which corresponds to the collected optical beam; and applying a low pass filter to the signal to digitally remove a plurality of problematic parasitic reflections located within the signal that represents an optical resonance, wherein the low pass filter filters out the parasitic reflections caused by the optical beam which passes through a substrate of the biosensor and is then reflected from a top surface of the biosensor, and wherein the low pass filter does not filter out the parasitic reflections caused by a part of the optical beam reflected from a face of the substrate of the biosensor. - View Dependent Claims (22)
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Specification