Method for detecting recombinant event

• US 7,981,607 B2
• Filed: 08/29/2005
• Issued: 07/19/2011
• Est. Priority Date: 08/27/2004
• Status: Active Grant

• Current Assignee: Esoterix Genetic Laboratories LLC (Laboratory Corporation Of America Holdings)
• Original Assignee: Esoterix Genetic Laboratories LLC (Laboratory Corporation Of America Holdings)
• Inventors: Shuber, Anthony P.
• Primary Examiner(s): Martinell; James
• Application Number:
  US11/661,528
• Publication Number:
  US 20090170077A1
• Time in Patent Office: 2,150 Days
• Field of Search: None
• US Class Current: 435/6.12
• CPC Class Codes: C12Q 1/6883 for diseases caused by alterations of genetic material

Abstract

Methods relating to isolating and amplifying chimeric nucleic acid molecules are provided. The methods of the invention are useful for detecting chromosome translocation events associated with diseases or conditions, such as cancer.

Claims

1. A method for detecting a chromosome translocation event in a DNA sample comprising

adding a DNA sample to an immobilized DNA capture probe capable of hybridizing to said DNA sample, wherein said DNA sample is repeatedly exposed to the immobilized DNA capture probe using reverse-field electrophoresis;

removing the captured DNA sample from the DNA capture probe and amplifying the captured DNA sample.

2. The method of claim 1, wherein the DNA capture probe is immobilized on one or more beads.

3. The method of claim 1, wherein two or more DNA capture probes are immobilized on a gel associated with a membrane.

4. The method of claim 1, wherein two or more DNA capture probes are immobilized.

5. The method of claim 1, wherein the captured DNA sample is amplified using a PCR reaction.

6. The method of claim 5, wherein a first primer and a second primer are present in the PCR reaction.

7. The method of claim 6, wherein the first primer corresponds to a region of a first chromosome.

8. The method of claim 6, wherein the second primer corresponds to a region of a second chromosome.

9. The method of claim 1, wherein the DNA sample is obtained from chorionic villus, placental biopsies, amniotic fluid, fetal blood, fetal serum, fetal plasma, maternal blood, maternal serum, or maternal plasma.