Supports for assaying analytes and methods of making and using thereof

• US 7,981,665 B2
• Filed: 01/22/2009
• Issued: 07/19/2011
• Est. Priority Date: 12/29/2005
• Status: Expired due to Fees

• Current Assignee: Corning Incorporated
• Original Assignee: Corning Incorporated
• Inventors: Frutos, Anthony G., Henry, David
• Primary Examiner(s): Haq; Shafiqul
• Application Number:
  US12/357,872
• Publication Number:
  US 20090137425A1
• Time in Patent Office: 908 Days
• Field of Search: None
• US Class Current: 435/287.2
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/0063 Other, e.g. van der Waals forces, hydrogen bonding
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00641 the porous medium being continuous, e.g. porous oxide substrates
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/0072 Organic compounds
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01J 2219/00731 Saccharides
• CPC Class Codes: B01J 2219/00736 Non-biologic macromolecules, e.g. polymeric compounds
• CPC Class Codes: B01J 2219/0074 Biological products
• CPC Class Codes: C08F 210/02 Ethene
• CPC Class Codes: C08F 222/06 Maleic anhydride
• CPC Class Codes: C08F 8/00 Chemical modification by after-treatment graft polymers, block polymers, crosslinking with unsaturated monomers or with polymers C08F251/00 - C08F299/00; of conjugated diene rubbers C08C
• CPC Class Codes: C08F 8/32 by reaction with amines
• CPC Class Codes: G01N 33/54353 : with ligand attached to the carrier via a chemical coupling agent coatings G01N33/54393
• CPC Class Codes: Y10T 436/143333 : Saccharide [e.g., DNA, etc.]

Abstract

Described herein are supports for assaying an analyte and methods of making and using thereof.

Claims

1. A method for making a support for performing an assay, comprising:

pre-blocking a binding polymer to provide a pre-blocked binding polymer of formula (I);

attaching the pre-blocked binding polymer of the formula (I) directly or indirectly to a substrate;

2. The method of claim 1, wherein the pre-blocking is accomplished with a nucleophilic group.

3. The method of claim 1, wherein the pre-blocking is accomplished with an alkyl amine, an alkylhydroxy amine, an alkoxyalkyl amine, an alcohol, an alkyl thiol, water, or H₂S.

4. The method of claim 1, wherein the pre-blocking is accomplished with water, ammonia, 2-(2-aminoethoxy)ethanol, N,N-dimethyl ethylenediamine, ethanolamine, ethylenediamine, hydroxyl amine, methoxyethyl amine, ethyl amine, isopropyl amine, butyl amine, propyl amine, hexyl amine, 2-amino-2-methyl-1-propanol, 2-(2-aminoethyl amino)ethanol, 2-(2-aminoethoxy)ethanol, dimethylethanolamine, dibutyl ethanolamine, 1-amino-2-propanol, polyethylene glycol, polypropylene glycol, 4,7,10-trioxa-1,13-tridecanediamine, polyethylene glycol or an amine-terminated-polyethylene glycol, Trizma hydrochloride, or any combination thereof.

5. The method of claim 1, wherein the pre-blocking is accomplished with methoxyethyl amine.

6. The method of claim 1, wherein the substrate comprises a plastic, a polymeric or co-polymeric substance, a ceramic, a glass, a metal, a crystalline material, a noble or semi-noble metal, a metallic or non-metallic oxide, an inorganic oxide, an inorganic nitride, a transition metal, or any combination thereof.

7. The method of claim 1, wherein the substrate comprises gold.

8. The method of claim 1, wherein the substrate comprises SiO₂ with a layer comprising Ta₂O₅, Nb₂O₅, TiO₂, Al₂O₃, silicon nitride or a mixture thereof, wherein the layer is adjacent to the surface of the SiO₂.

9. The method of claim 1, wherein the binding polymer comprises poly(vinyl acetate-maleic anhydride), polystyrene-co-maleic anhydride), poly(isobutylene-alt-maleic anhydride), poly(maleic anhydride-alt-1-octadecene), poly(maleic anhydride-alt-1-tetradecene), poly(maleic anhydride-alt-methyl vinyl ether), poly(triethyleneglycol methylvinyl ether-co-maleic anhydride), or any combination thereof.

10. The method of claim 1, wherein the binding polymer comprises poly(ethylene-alt-maleic anhydride).

11. The method of claim 1, wherein the ratio of reactive groups to inactive groups is from 0.5 to 5.0.

12. The method of claim 1, wherein the ratio of reactive groups to inactive groups is from 0.67 to 3.0.

13. The method of claim 1, wherein the substrate comprises a gold chip, the binding polymer comprises poly(ethylene-alt-maleic anhydride) indirectly attached to the substrate by an aminothiol, and the ratio of reactive groups to inactive groups in the binding polymer is from 0.67 to 3.0.

14. The method of claim 13, wherein prior to attachment to the substrate, the binding polymer is pre-blocked with methoxyethyl amine.

15. The method of claim 1, wherein the substrate comprises a glass substrate with a layer comprising Ta₂O₅, Nb₂O₅, TiO₂, Al₂O₃, silicon nitride, SiO₂ or a mixture thereof, the binding polymer comprises poly(ethylene-alt-maleic anhydride) indirectly attached to the substrate by a tie layer, wherein the tie layer is derived from aminopropylsilane, and the ratio of reactive groups to inactive groups in the binding polymer is from 0.67 to 3.0.

16. The method of claim 15, wherein prior to attachment to the substrate, the binding polymer is pre-blocked with methoxyethyl amine.

18. A support made by the method of claim 1.

20. The support of claim 18 wherein the substrate comprises a cell culture surface.

21. The support of claim 18 wherein the assay comprises a cell based assay.

22. The support of claim 18 wherein the biomolecule comprises a cell.

17. A method for making a support for performing an assay, comprising:

attaching a pre-blocked binding polymer of the formula (I) directly or indirectly to a substrate;

19. A support made by the method of claim 17.