Generation of acyl amino acids

• US 7,981,685 B2
• Filed: 04/16/2008
• Issued: 07/19/2011
• Est. Priority Date: 04/16/2007
• Status: Active Grant

• Current Assignee: Modular Genetics, Inc.
• Original Assignee: Modular Genetics, Inc.
• Inventors: Reznik, Gabriel, Vishwanath, Prashanth, Jarrell, Kevin A.
• Primary Examiner(s): JOIKE, MICHELE K
• Application Number:
  US12/596,272
• Publication Number:
  US 20110030102A1
• Time in Patent Office: 1,189 Days
• Field of Search: None
• US Class Current: 436/112
• CPC Class Codes: C12N 15/62 DNA sequences coding for fusion proteins
• CPC Class Codes: C12N 15/8243 involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
• CPC Class Codes: C12N 15/8251 Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
• CPC Class Codes: C12N 15/8257 for the production of primary gene products, e.g. pharmaceutical products, interferon
• CPC Class Codes: C12P 13/02 Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes peptides C12P21/00 or C07K
• CPC Class Codes: C12P 13/04 Alpha- or beta- amino acids other amino acids C12P13/005
• CPC Class Codes: C12P 13/14 Glutamic acid; Glutamine
• CPC Class Codes: Y10T 436/174614 Tertiary amine

Abstract

Engineered polypeptides useful in synthesizing acyl amino acids are provided. Also provided are methods of making acyl amino acids using engineered polypeptides. In certain embodiments, an acyl amino acid produced using compositions and/or methods of the present invention comprises cocoyl glutamate.

Claims

1. A composition comprising an acyl amino acid isolated from a bacterial cell, wherein the acyl amino acid comprises a single natural glutamic acid covalently linked to a natural fatty acid, wherein the natural fatty acid is a beta-hydroxy fatty acid with a branched carbon chain.

2. The composition of claim 1, wherein the natural fatty acid is produced by the bacterial cell and wherein the composition is free of bacterial cell proteins.

3. The composition of claim 1, wherein the natural fatty acid is beta-hydroxy myristic acid.