Method of lipid structure preparation

• US 7,985,417 B2
• Filed: 10/07/2005
• Issued: 07/26/2011
• Est. Priority Date: 10/08/2004
• Status: Expired due to Fees

• Current Assignee: Yissum Research Development Company of the Hebrew University of Jerusalem Ltd. (Hebrew University of Jerusalem)
• Original Assignee: Yissum Research Development Company of the Hebrew University of Jerusalem Ltd. (Hebrew University of Jerusalem)
• Inventors: Garbuzenko, Olga, Barenholz, Yechezkel
• Primary Examiner(s): Krass; Frederick
• Assistant Examiner(s): Shomer; Isaac
• Application Number:
  US11/246,340
• Publication Number:
  US 20060121105A1
• Time in Patent Office: 2,118 Days
• Field of Search: None
• US Class Current: 424/450
• CPC Class Codes: A61K 47/6907 the form being a microemulsion, nanoemulsion or micelle
• CPC Class Codes: A61K 47/6913 the liposome being modified on its surface by an antibody
• CPC Class Codes: A61K 9/1271 Non-conventional liposomes, e.g. PEGylated liposomes, liposomes coated with polymers liposome as conjugate A61K47/6911
• CPC Class Codes: A61K 9/1277 Processes for preparing; Proliposomes
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: C07K 16/32 against translation products of oncogenes
• CPC Class Codes: C07K 2317/622 Single chain antibody (scFv)

Abstract

A method of inserting a lipid-linked moiety into a lipid assembly, such as a planar lipid monolayer or bilayer, a spherical lipid vesicle, a micelle, or an emulsion envelope monolayer is described. In the method, the lipid assembly and the lipid-linked moiety are contacted in the presence of microwave irradiation to permit the lipid-linked moiety to become associated with the lipid assembly. In one embodiment, the lipid assembly is a liposome and the lipid-linked moiety is a lipid-polymer. Compositions comprised of a lipid layer and of a lipid-linked moiety, prepared in accord with the method, are also described.

Claims

1. A method for inserting a lipid-linked moiety into a lipid assembly, comprising:

contacting a lipid assembly comprising HSPC, cholesterol, EPC or mPEG-DSPE and a lipid-linked moiety comprising a DSPE-PEG conjugate in the presence of 510W of microwave energy for between 30 seconds and 10 minutes, thereby inserting the lipid linked moiety into a lipid assembly, wherein said lipid assembly is selected from the group consisting of a lipid monolayer, a lipid bilayer, a liposome, a micelle, or an emulsion envelope monolayer.

2. The method of claim 1, wherein said lipid assembly comprises an entrapped therapeutic agent.