Biochemical reaction cartridge

• US 7,988,913 B2
• Filed: 10/13/2006
• Issued: 08/02/2011
• Est. Priority Date: 03/31/2003
• Status: Expired due to Fees

• Current Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Original Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Inventors: Numajiri, Yasuyuki
• Primary Examiner(s): JARRETT, LORE RAMILLANO
• Application Number:
  US11/549,204
• Publication Number:
  US 20070071637A1
• Time in Patent Office: 1,754 Days
• Field of Search: 422/57, 422/58, 422/65, 422/401, 435/288.4
• US Class Current: 422/65
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2300/1822 using Peltier elements
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502761 specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046

Abstract

A biochemical reaction cartridge includes an injection port for injecting a specimen, a chamber for containing the specimen therein, a chamber for containing a reagent for treating the specimen, nozzle ports for applying or reducing pressure by using fluid. In the cartridge, the specimen is subjected to a biochemical reaction by controlling the fluid. The cartridge is mounted in a biochemical reaction apparatus.

Claims

1. A biochemical reaction cartridge, comprising:

a storage chamber for accumulating a liquid containing a reagent,

a first function chamber, having a specimen port, for mixing a predetermined amount of the liquid with a predetermined amount of a specimen,

a second function chamber, for reciprocating the liquid mixed with the specimen in the first function chamber,

a first passage for connecting a bottom of the storage chamber to the first function chamber to move the liquid from the storage chamber to the first function chamber,

a second passage for connecting a bottom of the first function chamber to a bottom of the second function chamber to reciprocally move the liquid between the first function chamber and the second function chamber,

a first connecting portion, for connecting the first function chamber to the first passage, having a bottom position, and

a second connecting portion, for connecting the first function chamber to the second passage, having a bottom position,

wherein the first passage has a connecting portion, for connecting the first passage to the first function chamber, having a height greater than a liquid surface of the liquid mixed with the specimen in the first function chamber.

2. The cartridge according to claim 1, wherein the cartridge comprises a pressure reducing portion for externally reducing the pressure, the pressure reducing portion being provided with a chamber for preventing outflow of the liquid.

3. The cartridge according to claim 1, wherein the cartridge further comprises a plurality of nozzle ports for receiving a plurality of nozzles for applying or reducing pressure, the plurality of nozzle ports communicating with the first or second function chamber, and wherein fluid is present between the plurality of nozzle ports and the first or second function chamber and is pressurized or depressurized by the plurality of nozzles to move the specimen and/or the reagent and/or the reaction liquid, thereby to effect a sequence of a biochemical reaction within the cartridge.

4. The cartridge according to claim 3, wherein the plurality of nozzle ports are divided into two portions, which are disposed on two surfaces of the cartridge, and the cartridge is a substantially rectangular parallelepiped, and the two surfaces are opposite lateral surfaces of the substantially rectangular parallelepiped.

5. The cartridge according to claim 1, wherein a bottom position of a connecting portion for connecting the first passage to the first function chamber is different from a bottom position of the storage chamber and a bottom position of the first connecting passage, and a bottom position of another connecting portion for connecting the second passage to the first function chamber is different from a bottom position of the first function chamber and a bottom position of the second passage.

6. The cartridge according to claim 1, wherein the chambers and the passages are formed with a plurality of members, which have been laminated.

7. A biochemical reaction cartridge, comprising:

a storage chamber for accumulating a liquid containing a reagent,

a first function chamber, having a specimen port, for mixing a predetermined amount of the liquid with a predetermined amount of a specimen,

a second function chamber, for reciprocating the liquid mixed with the specimen in the first function chamber,

a first passage for connecting a bottom of the storage chamber to the first function chamber to move the liquid from the storage chamber to the first function chamber, and

a second passage for connecting a bottom of the first function chamber to a bottom of the second function chamber to reciprocally move the liquid between the first function chamber and the second function chamber,

wherein the first passage has a first connecting portion, for connecting the first passage to the first function chamber, having a height greater than a liquid surface of the liquid mixed with the specimen in the first function chamber, and

wherein the second passage has a second connecting portion, for connecting the second passage to the first function chamber, having a height smaller than the liquid surface of the liquid mixed with the specimen in the first function chamber.