Handheld and portable microfluidic device to automatically prepare nucleic acids for analysis

• US 7,988,935 B2
• Filed: 09/09/2005
• Issued: 08/02/2011
• Est. Priority Date: 09/09/2004
• Status: Expired due to Fees

• Current Assignee: MicroFluidic Systems, Inc.
• Original Assignee: MicroFluidic Systems, Inc.
• Inventors: Aflaatooni, Nima, Yuan, Bob
• Primary Examiner(s): Gordon; Brian R
• Application Number:
  US11/223,095
• Publication Number:
  US 20060051252A1
• Time in Patent Office: 2,153 Days
• Field of Search: 422100-101, 422501-502, 422/513, 422/527, 422534-535, 436/180, 738/633.2, 738/64, 738/640.1
• US Class Current: 422/527
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 2400/0478 pistons
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/0217 of the plunger pump type medical syringes A61M
• CPC Class Codes: B01L 3/0293 for liquids
• CPC Class Codes: B01L 3/502 with fluid transport, e.g. in multi-compartment structures centrifugal-type cuvettes G01N21/07; analysis by separation into components G01N30/00; automatic analysers G01N35/00
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 9/527 for microfluidic devices, e.g. used for lab-on-a-chip
• CPC Class Codes: G01N 1/14 Suction devices, e.g. pumps; Ejector devices
• CPC Class Codes: G01N 1/40 Concentrating samples
• CPC Class Codes: G01N 1/405 by adsorption or absorption

Abstract

A handheld and portable extraction device is directed to a microfluidic-based system to be used in the field to extract and purify an analyte, preferably a nucleic acid, from a fluid-based sample. Preferably, the fluid-based sample is water-based. The fluid-based sample can also be a biological fluid sample. The handheld and portable extraction device includes a syringe-like device coupled to a purification chip. The purification chip is preferably included within a chip block which is removable from the remaining portion of the handheld and portable extraction device. The analyte collected within the purification chip can be later removed and collected for analysis.

Claims

1. A handheld and portable device comprising:

a. a device input opening to the handheld and portable device, the device input opening is configured to provide external access and to input an external fluid-based sample into the handheld and portable device;

b. a single syringe-like apparatus, wherein the syringe-like apparatus is configured for drawing the fluid-based sample into the device, wherein the syringe-like apparatus includes means for storing the sample input into the device;

c. first means for directing coupled to the device input opening and to the means for storing, wherein the first means for directing is configured to transport the sample from the device input opening to the means for storing to input the sample into the means for storing;

d. means for extracting and purifying an analyte from the sample, wherein the means for extracting and purifying includes a purification chip having a plurality of pillars, each pillar coated with silicon oxide;

e. second means for directing coupled to the means for storing and to the means for extracting and purifying, wherein the second means for directing is configured to transport the sample from the means for storing into the means for extracting and purifying, further wherein the first means for directing, the means for storing, the second means for directing, and the means for extracting and purifying are configured to transport the sample into the means for storing prior to the sample entering the means for extracting and purifying; and

f. means for regulating coupled to the means for storing, wherein the means for regulating is configured to regulate a fluid flow rate of the sample through the means for extracting and purifying.

2. The handheld and portable device of claim 1 wherein the syringe-like apparatus further comprises a plunger coupled to the means for storing, and a pipette tip coupled to the device input opening and to the means for storing, and the means for storing comprises a sample collection chamber.

3. The handheld and portable device of claim 2 wherein the means for regulating includes a spring coupled to the plunger.

4. The handheld and portable device of claim 2 wherein the means for regulating includes one of a group consisting of a pump, a motor, or a CO₂ pressure canister.

5. The handheld and portable device of claim 1 further comprising means for separating to separate solid debris from the fluid-based sample, wherein the means for separating is coupled to the means for drawing and the means for directing.

6. The handheld and portable device of claim 5 wherein the means for separating includes one or more filters.

7. The handheld and portable device of claim 1 wherein the first means for directing includes a first check valve and the second means for directing includes a second check valve.

8. The handheld and portable device of claim 1 wherein a density of the plurality of pillars is greater at an output port side of the purification chip than at an input port side of the purification chip.

9. The handheld and portable device of claim 1 wherein the means for extracting and purifying further comprises a chip block including the purification chip and microfluidic pathways coupled to and from the purification chip, wherein the microfluidic pathway coupled to the purification chip is also coupled to the means for directing.

10. The handheld and portable device of claim 9 wherein the chip block is detachable from the handheld and portable device.

11. The handheld and portable device of claim 7 further comprising a tee-junction having a first fluid port, a second fluid port, and a third fluid port, wherein the first fluid port is coupled to the first check valve, the second fluid port is coupled to the means for storing, and the third fluid port is coupled to the second check valve.

12. The handheld and portable device of claim 11 wherein a portion of the tee junction is included in and common to both the first means for directing and the second means for directing.

13. The handheld and portable device of claim 11 wherein the second means for directing includes a connector having a fluid conduit extending through the connector from a first end to a second end, wherein the first end is coupled to the second check valve and the second end is coupled to the means for extracting and purifying.

14. The handheld and portable device of claim 13 further comprising a pipette tip coupled to the first check valve, wherein the pipette tip includes the device input opening.

15. A handheld and portable device comprising:

a. a device input opening to the handheld and portable device, the device input opening is configured to provide external access and to input an external fluid-based sample into the handheld and portable device;

b. a syringe-like device to draw the fluid-based sample into the device, wherein the syringe-like device includes a sample collection chamber to store the sample;

c. a first fluidic pathway coupled to the device input opening and to the sample collection chamber to transport the sample from the device input opening into the sample collection chamber, wherein the first fluidic pathway includes a first check valve;

d. a purification chip to extract and purify an analyte from the sample;

e. a second fluidic pathway coupled to the sample collection chamber and to the purification chip to transport the sample from the sample collection chamber into the purification chip, wherein the first fluidic pathway, the sample collection chamber, the second fluidic pathway, and the purification chip are configured to transport the sample into the sample collection chamber prior to the sample entering the purification chip, wherein the second fluidic pathway includes a second check valve; and

f. a fluid flow regulator coupled to the syringe-like device to regulate a fluid flow rate of the sample through the purification chip.

16. The handheld and portable device of claim 15 wherein the syringe-like device further includes a plunger coupled to the sample collection chamber and a pipette tip coupled to the device input opening and the sample collection chamber.

17. The handheld and portable device of claim 16 wherein the fluid flow regulator includes a spring coupled to the plunger.

18. The handheld and portable device of claim 16 wherein the fluid flow regulator includes one of a group consisting of a pump, a motor, or a CO₂ pressure canister.

19. The handheld and portable device of claim 15 further comprising a separator to separate solid debris from the fluid-based sample, wherein the separator is coupled to the syringe-like device and the fluid flow regulator.

20. The handheld and portable device of claim 19 wherein the separator includes one or more filters.

21. The handheld and portable device of claim 15 wherein the purification chip includes a plurality of pillars, each pillar coated with silicon oxide.

22. The handheld and portable device of claim 21 wherein a density of the plurality of pillars is greater at an output port side of the purification chip than at an input port side of the purification chip.

23. The handheld and portable device of claim 15 further comprises a chip block including the purification chip and microfluidic pathways coupled to and from the purification chip, wherein the microfluidic pathway coupled to the purification chip is also coupled to the second fluidic pathway.

24. The handheld and portable device of claim 23 wherein the chip block is detachable from the handheld and portable device.

25. The handheld and portable device of claim 15 further comprising a tee-junction having a first fluid port, a second fluid port, and a third fluid port, wherein the first fluid port is coupled to the first check valve, the second fluid port is coupled to the sample collection chamber, and the third fluid port is coupled to the second check valve.

26. The handheld and portable device of claim 25 wherein a portion of the tee junction is included in and common to both the first fluidic pathway and the second fluidic pathway.

27. The handheld and portable device of claim 25 wherein the second fluidic pathway includes a connector having a fluid conduit extending through the connector from a first end to a second end, wherein the first end is coupled to the second check valve and the second end is coupled to the purification chip.

28. The handheld and portable device of claim 27 further comprising a pipette tip coupled to the first check valve, wherein the pipette tip includes the device input opening.