RGD polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid) / β tricalcium phosphate composite material and preparation method thereof

• US 7,989,532 B2
• Filed: 07/10/2008
• Issued: 08/02/2011
• Est. Priority Date: 06/29/2006
• Status: Expired due to Fees

• Current Assignee: Wuhan University of Technology
• Original Assignee: Wuhan University of Technology
• Inventors: Yan, Yuhua, Dai, Honglian, Wan, Tao, Wang, Xinyu, Li, Shipu, Yan, Qiongjiao, Yuan, Lin, Han, Yingchao
• Primary Examiner(s): Eashoo; Mark
• Assistant Examiner(s): HEINCER, LIAM J
• Application Number:
  US12/170,643
• Publication Number:
  US 20080319114A1
• Time in Patent Office: 1,118 Days
• Field of Search: 524/417, 524/436, 525/54.11
• US Class Current: 524/417
• CPC Class Codes: A61L 2300/252 Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
• CPC Class Codes: A61L 2430/32 for nerve reconstruction
• CPC Class Codes: A61L 27/18 obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
• CPC Class Codes: A61L 27/227 Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
• CPC Class Codes: A61L 27/46 with phosphorus-containing inorganic fillers
• CPC Class Codes: A61L 27/54 Biologically active materials, e.g. therapeutic substances A61L27/227 takes precedence
• CPC Class Codes: C07K 14/75 Fibrinogen
• CPC Class Codes: C07K 14/78 Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
• CPC Class Codes: C08L 67/04 Polyesters derived from hydroxycarboxylic acids, e.g. lactones C08L67/06 takes precedence

Abstract

An RGD polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid)/β-tricalcium phosphate composite material is composed of β-tricalcium phosphate particles and RGD polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid) with mass ratio of 1:10-1:100, in which the β-tricalcium phosphate particles are uniformly dispersed in the RGD polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid) matrix. The preparation method includes that poly (glycolic acid-L-lysine-L-lactic acid) is polymerized with GRGDY short peptide (glycin-arginine-glycin-aspartic acid-tyrosine sequence) to obtain RGD polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid), and then RGD polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid) is compounded with β-tricalcium phosphate particles. The composite material exhibits favorable biocompatibility, cellular affinity, biodegradability and mechanical behavior, and can avoid aseptic necrosis of tissues, which may be used as nerve guide or porous bone scaffold for repairing nerve tissue and bone tissue.

Claims

1. An RGD polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid)/β

-tricalcium phosphate composite material, comprising a two-phase mixture of β

-tricalcium phosphate particles and RGD polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid), the RGD polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid) consisting of the following molecular structure;

2. The RGD polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid)/β

-tricalcium phosphate composite material according to claim 1, wherein said particle size of β

-tricalcium phosphate particles is between 0.05 μ

m and 2 μ

m.

3. The material of claim 2, wherein the mass ratio of β

-tricalcium phosphate particles and RGD polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid) is between 1;

10 and 1;

50.

4. The RGD polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid)/β

-tricalcium phosphate composite material according to claim 1, wherein the mass ratio of β

-tricalcium phosphate particles and RGD polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid) is between 1;

10 and 1;

50.

5. The polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid)/β

-tricalcium phosphate composite material of claim 1, wherein the particle size of β

-tricalcium phosphate particles is from 0.05 μ

m up to but not including 1 μ

m.

6. The polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid)/β

-tricalcium phosphate composite material of claim 1, wherein the particle size of β

-tricalcium phosphate particles is less than 1 μ

m.

7. The polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid)/β

-tricalcium phosphate composite material of claim 1, wherein the number average molecular weight of RGD polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid) is 50,000-250,000.

8. The polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid)/β

-tricalcium phosphate composite material of claim 1, wherein the number average molecular weight of RGD polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid) is 80,000-300,000.

9. The polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid)/β

-tricalcium phosphate composite material of claim 1, wherein the polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid)/β

-tricalcium phosphate composite material is provided in the form of a nerve guide for repairing nerve tissue.

10. The polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid)/β

-tricalcium phosphate composite material of claim 1, wherein the polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid)/β

-tricalcium phosphate composite material is provided in the form of a porous bone scaffold for repairing bone tissue.

11. An RGD polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid)/β

-tricalcium phosphate composite material, comprising a two-phase mixture of;

(i) β

-tricalcium phosphate particles, and (ii) RGD polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid), the RGD polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid) consisting of the following molecular structure;

12. The polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid)/β

-tricalcium phosphate composite material of claim 11, wherein the particle size of β

-tricalcium phosphate particles is from 0.05 μ

m up to but not including 1 μ

m.

13. An RGD polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid)/β

-tricalcium phosphate composite material, comprising a two-phase mixture of;

(i) β

-tricalcium phosphate particles, and (ii) RGD polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid), the RGD polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid) consisting of the following molecular structure;

14. The RGD polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid)/β

-tricalcium phosphate composite material of claim 13, wherein X;

Y is between 1;

10 and 1;

100.

15. The polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid)/β

-tricalcium phosphate composite material of claim 13, wherein the particle size of β

-tricalcium phosphate particles is from 0.05 μ

m up to but not including 1 μ

m.